Moderate to high ethanol and acetaldehyde administration decreases c-fos protein expression in the hippocampus of Aldh2-knockout and C57BL/6N mice

Authors

  • Mostofa Jamal Assistant Professor, Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1, Ikenobe, Miki, Kita, Kagawa 761-0793, Japan
  • Ayako Ito Assistant Professor, Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1, Ikenobe, Miki, Kita, Kagawa 761-0793, Japan
  • Naoko Tanaka Lecturer, Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1, Ikenobe, Miki, Kita, Kagawa 761-0793, Japan
  • Kiyoshi Ameno Associate Professor, Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1, Ikenobe, Miki, Kita, Kagawa 761-0793, Japan
  • Takanori Miki Professor, Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Japan
  • Hiroshi Kinoshita Professor, Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1, Ikenobe, Miki, Kita, Kagawa 761-0793, Japan

DOI:

https://doi.org/10.3329/bjms.v19i1.43876

Keywords:

Aldh2-KO mice; ethanol; acetaldehyde; c-fos protein; hippocampus

Abstract

Background: Ethanol (EtOH) and acetaldehyde (AcH) have long been associated with many adverse effects in the central nervous system. c-fos has been used as an indirect index of neural activity.

Method: Here, we investigated the effects of systemic administration of EtOH and AcH on the expression of c-fos protein in the hippocampus of Aldh2-knockout (Aldh2-KO) mice. The animals received an intraperitoneal injection of saline (control), EtOH (1.0, 2.0 and 4.0 g/kg) or AcH (50, 100 and 200 mg/kg), and the expression of c-fos protein was measured by Western blotting.

Result: We found that EtOH at doses of 2.0 and 4.0 g/kg decreased c-fos in wild-type (WT) mice, whereas EtOH at all three doses tested (1.0-4.0 g/kg) decreased c-fos in Aldh 2-KO mice. Likewise, AcH at doses of 100 and 200 mg/kg had a significant effect in lowering c-fos protein in both WT and Aldh2-KO mice.

Conclusion: Our observations suggest that moderate to high EtOH and AcH can decrease the expression of c-fos protein in the mouse hippocampus. This effect may support, at least in part, the link between c-fos and spatial memory deficits following EtOH and AcH exposure as we have observed in our previous study.

Bangladesh Journal of Medical Science Vol.19(1) 2020 p.83-89

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Published

2019-11-03

How to Cite

Jamal, M., Ito, A., Tanaka, N., Ameno, K., Miki, T., & Kinoshita, H. (2019). Moderate to high ethanol and acetaldehyde administration decreases c-fos protein expression in the hippocampus of Aldh2-knockout and C57BL/6N mice. Bangladesh Journal of Medical Science, 19(1), 83–89. https://doi.org/10.3329/bjms.v19i1.43876

Issue

Vol. 19 No. 1 (2020)

Section

Original Articles

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