Safety evaluation of human peripheral blood mononuclear cells in naive rats:a chronic toxicitystudy

Authors

  • Basuki Supartono Stem Cell and Tissue Engineering Research Center, Faculty of Medicine, UPN Veteran Jakarta, Indonesia
  • Siti Farida Departement of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia, Indonesia
  • Sony Suhandono Schoolof Life Science and Technology Institut Teknologi Bandung
  • Ahmad Aulia Yusuf Histology Departement, Faculty of Medicine Universitas Indonesia, Indonesia. Stemcell and Tissue Engineering Indonesian Medical Education and Research Institute

DOI:

https://doi.org/10.3329/bjms.v21i2.57029

Keywords:

Human peripheral blood mononuclear cells, Chronic toxicity, Naive rat, Tissue engineering, Stemcell transplantation

Abstract

Human peripheral blood mononuclear cells (PBMC) are widely used within tissue engineering therapy, thus, the evaluation of their safety is mandatory. The present study aims to assess the safety of human PBMC transplantations in naive rats in terms of chronic toxicity. Rats received intravenous injections of human PBMC with doses of 105, 106, or 107 cells (n = 5/group/sex) every month for three months without administration of any immunosuppressant drugs. We evaluated clinical, physical, laboratory, and pathology parameters. No morbidity, mortality, or significant differences occurred within all of the tested parameters between control and experimental groups until the finalization of the study. In conclusion, the current work indicates that human PBMC transplantation in naive rats did not induce chronic toxicity reaction.

Bangladesh Journal of Medical Science Vol. 21 No. 02 April’22 Page : 373-383

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Published

2022-02-25

How to Cite

Supartono, B. ., Farida, S. ., Suhandono, S. ., & Yusuf, A. A. . (2022). Safety evaluation of human peripheral blood mononuclear cells in naive rats:a chronic toxicitystudy. Bangladesh Journal of Medical Science, 21(2), 373–383. https://doi.org/10.3329/bjms.v21i2.57029

Issue

Vol. 21 No. 2 (2022)

Section

Original Articles

License

Copyright (c) 2022 Basuki Supartono, Siti Farida, Sony Suhandono, Ahmad Aulia Yusuf

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