Mesenchymal Stem Cells Combined with Bovine Colostrum Decrease the PDGF and Fibrosis Level after Hepatectomy in Liver Fibrosis Rats Model

Authors

  • Ahmad Fathi Fuadi Trainee of Digestive Surgery Department, Kariadi Hospital-Faculty of Medicine, Diponegoro University, Semarang, 50244, Indonesia https://orcid.org/0000-0003-1647-4190
  • Ignatius Riwanto Digestive Surgery Department, Kariadi Hospital-Faculty of Medicine, Diponegoro University, Semarang, 50244, Indonesia
  • Agung Putra Stem Cells and Cancer Research, Department of Pathological Anatomy and Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia https://orcid.org/0000-0003-4261-9437
  • Erik Prabowo Digestive Surgery Department, Kariadi Hospital-Faculty of Medicine, Diponegoro University, Semarang, 50244, Indonesia https://orcid.org/0000-0002-0379-2328
  • Hermawan Istiadi Pathology Anatomy Department, Kariadi Hospital-Faculty of Medicine, Diponegoro University, Semarang, 50244, Indonesia https://orcid.org/0000-0002-6391-3830

DOI:

https://doi.org/10.3329/bjms.v21i2.58057

Keywords:

Mesenchymal Stem Cell; Bovine Colostrum; Liver fibrosis; Hepatectomy

Abstract

Introduction: Liver fibrosis is a healing response of the liver to injury by depositing excessive extracellular matrix proteins. Although this fibrosis has a strong impact on survival following resection, there is no therapeutic guideline so far for liver fibrosis. Platelet-derived Growth Factor (PDGF) is the most potent mitogen of Hepatic Stellate Cells (HSC) which started liver fibrogenesis. Mesenchymal Stem Cells (MSCs) have an anti-fibrotic effect by controlling inflammation and HSC immunomodulation, while Bovine Colostrum (BC) has an antifibrotic effect by its antioxidant capacity and growth factor content. However, their effect was only studied in the non-resected fibrotic liver and their combination is never been studied. our study aimed to examine the effects of MSC, BC and their combination on PDGF and fibrosis level in the fibrotic liver after resection.

Material and methods: Twenty-five Sprague-Dawley rats with fibrotic liver were randomly assigned to BC, MSC, MSC+BC, control, and sham groups. Hepatectomy 50% was carried out except for the sham group. The 106MSCs were given intraparenchymal liver, while BC (15 μL/g) was given orally for 5 days/week until day 10. The PDGF plasma level was assessed on days 3, 7, and 10. The histopathologic examination of the liver remnant was done on day 10 using Metavir Scoring System.

Result: MSC+BC significantly increased PDGF level on day 3 followed by a significant decrease on day 10 (p<0.001). The Metavir mean score was 1.2 in the MSC+BC group.

Conclusion: MSC+BC combination can improve liver fibrosis after hepatectomy of liver fibrosis rats by preventing post-hepatectomy fibrogenesis.

Bangladesh Journal of Medical Science Vol. 21 No. 02 April’22 Page : 262-270

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Published

2022-02-25

How to Cite

Fuadi, A. F. ., Riwanto, I. ., Putra, A. ., Prabowo, E. ., & Istiadi, H. . (2022). Mesenchymal Stem Cells Combined with Bovine Colostrum Decrease the PDGF and Fibrosis Level after Hepatectomy in Liver Fibrosis Rats Model. Bangladesh Journal of Medical Science, 21(2), 262–270. https://doi.org/10.3329/bjms.v21i2.58057

Issue

Vol. 21 No. 2 (2022)

Section

Original Articles

License

Copyright (c) 2022 Ahmad Fathi Fuadi, Ignatius Riwanto, Agung Putra, Erik Prabowo, Hermawan Istiadi

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