Installation of AutoDELFIA: A new addition in Bangladesh for the Screening of Congenital Hypothyroidism in Newborn Babies

Authors

  • Sanchoy Chandra Biswasarma In vitro Division,National Institute of Nuclear Medicine and Allied Sciences (NINMAS), BSMMU, Dhaka
  • Md Ariful Islam In vitro Division,National Institute of Nuclear Medicine and Allied Sciences (NINMAS), BSMMU, Dhaka
  • Mohammad Anwar ul Azim In vitro Division,National Institute of Nuclear Medicine and Allied Sciences (NINMAS), BSMMU, Dhaka
  • Md Jashim Uddin In vitro Division,National Institute of Nuclear Medicine and Allied Sciences (NINMAS), BSMMU, Dhaka
  • Hasan Mehdi Medical Physics Division,National Institute of Nuclear Medicine and Allied Sciences (NINMAS), BSMMU, Dhaka
  • Md Shohag Mia Medical Physics Division,National Institute of Nuclear Medicine and Allied Sciences (NINMAS), BSMMU, Dhaka
  • Mustafa Mamun In vitro Division,National Institute of Nuclear Medicine and Allied Sciences (NINMAS), BSMMU, Dhaka
  • Md Alamgir Kabir In vitro Division,National Institute of Nuclear Medicine and Allied Sciences (NINMAS), BSMMU, Dhaka
  • Sakera Khatun In vitro Division,National Institute of Nuclear Medicine and Allied Sciences (NINMAS), BSMMU, Dhaka
  • Md Nahid Hossain Medical Physics Division,National Institute of Nuclear Medicine and Allied Sciences (NINMAS), BSMMU, Dhaka
  • Zeenat Jabin Thyroid Division, National Institute of Nuclear Medicine and Allied Sciences (NINMAS), Shahbag, Dhaka
  • Fatima Begum Thyroid Division, National Institute of Nuclear Medicine and Allied Sciences (NINMAS), Shahbag, Dhaka
  • Nurun Nahar Ex-Director,National Institute of Nuclear Medicine and Allied Sciences (NINMAS), Shahbag, Dhaka
  • Mizanul Hasan Ex-Director,National Institute of Nuclear Medicine and Allied Sciences (NINMAS), Shahbag, Dhaka
  • Jamiul Hossain Institute of Nuclear Medicine and Allied Sciences (NINMAS), Mitford, Dhaka

DOI:

https://doi.org/10.3329/bjnm.v23i1-2.57703

Keywords:

Newborn Screening, Congenital Hypothyroidism, Auto DELFIA, Limit of detection, Limit of Quantification

Abstract

Newborn Screening for the diagnosis of Congenital Hypothyroidism (CH) was first started in Bangladesh in 1999 by the Immunoradiometric assay (IRMA). Dissociation enhanced lanthanide fluorescent immunoassay (DELFIA) was started in 1986 due to its superior sensitivity, speed, and non-radioactive methodology. Auto DELFIA 1235 automatic immunoassay system was introduced in the In vitro Division of National Institute of Nuclear Medicine & Allied Sciences (NINMAS) under the ADP Project “Screening of Congenital Hypothyroidism in Newborn Babies (Phase 2)”. It is an automatic system that can minimize the pipetting error. In this experiment, dried blood spots (DBS) were punched into an antibody-coated microtitration well with an automated Panthera Puncher and analyzed by AutoDELFIA. Six different standards of 0.8, 10.3, 24.5, 49.3, 93.2, 239μU/mL were used and the calibration curve was drawn by using these standards. Standard error and Standard deviation were found 12.4 and 30.5. Limit of detection (LOD) and Limit of Quantification (LOQ) of X variables were 0.16 and 0.47. The average of the percent recovery was 96.7. The R2 value of the calibration curve was 0.9985. The accuracy of the standard samples was 96.7 ±10.6. For this assay two Quality Control (QC) of 16.39 μU/mL and 63.09, μU/mL were used. All of the measured values from the calibration curve were close to ±1 standard deviation. All these findings indicate appropriateness of Auto DELFIA System for the CH screening from dried blood spots in the newborn screening laboratory of the In vitro division of NINMAS.

Bangladesh J. Nuclear Med. 23(1&2): 15-20, 2020

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Published

2022-05-04

How to Cite

Biswasarma, S. C., Islam, M. A., ul Azim, M. A., Uddin, M. J. ., Mehdi, H. ., Mia, M. S. ., Mamun, M. ., Kabir, M. A. ., Khatun, S. ., Hossain, M. N. ., Jabin, Z. ., Begum, F. ., Nahar, N., Hasan, M. ., & Hossain, J. . (2022). Installation of AutoDELFIA: A new addition in Bangladesh for the Screening of Congenital Hypothyroidism in Newborn Babies. Bangladesh Journal of Nuclear Medicine, 23(1-2), 15–20. https://doi.org/10.3329/bjnm.v23i1-2.57703

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Original Articles