Effect of thymoquinone on cyclophosphamide-induced oxidative and inflammatory ovarian damage in rat
DOI:
https://doi.org/10.3329/bjp.v17i3.59418Keywords:
Cyclophosphamide, Ovarian damage, Thymoquinone, RatAbstract
This study intends to investigate the effect of thymoquinone against possible ovarian damage and infertility caused by cyclophosphamide in female rats. The rats in the experiment (n=36) were separated into three groups; cyclophosphamide administered, thymoquinone plus cyclophosphamide administered and healthy control. At the end of 30 days of administration, 6 rats from each group were killed and the biochemical and histopathological examina-tions were performed on the ovarian tissues. The remaining animals were kept in the same cage with mature male rats for two months. Infertility developed in 83% of cyclophosphamide group animals. Thymoquinone attenuated cyclophosphamide-related oxidative and inflammatory ovarian damage and reduced the development of infertility. The outcomes suggest that thymoquinone may be useful in treating infertility due to oxidative and inflammatory ovarian damage.
Downloads
43
32
References
Alenzi FQ , El-Bolkiny Yel S, Salem ML. Protective effects of Nigella sativa oil and thymoquinone against toxicity induced by the anticancer drug cyclophosphamide. Br J Biomed Sci. 2010; 67: 20-28.
Ali MY, Akter Z, Mei Z, Zheng M, Tania M, Khan MA. Thymoquinone in autoimmune diseases: Therapeutic potential and molecular mechanisms. Biomed Pharmacother. 2021; 134: 111157.
Anderson RA, Themmen AP, Al-Qahtani A, Groome NP, Cameron DA. The effects of chemotherapy and long-term gonadotrophin suppression on the ovarian reserve in pre-menopausal women with breast cancer. Hum Reprod. 2006; 21: 2583-92.
Blokhina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress: A review. Ann Bot. 2003; 91: 179-94.
Butt MS, Imran M, Imran A, Arshad MS, Saeed F, Gondal TA, Shariati MA, Gilani SA, Tufail T, Ahmad I, Rind NA. Therapeutic perspective of thymoquinone: A mechanistic treatise. Food Sci Nutr. 2021; 9: 1792-809.
Capuzzi E, Ossola P, Caldiroli A, Auxilia AM, Buoli M. Malondialdehyde as a candidate biomarker for bipolar disorder: A meta-analysis. Prog Neuropsychopharmacol Biol Psychiatry. 2022; 113: 110469.
Clarkson PM, Thompson HS. Antioxidants: What role do they play in physical activity and health? Am J Clin Nutr. 2000; 72: 637S-46S.
Darakhshan S, Bidmeshki Pour A, Hosseinzadeh Colagar A, Sisakhtnezhad S. Thymoquinone and its therapeutic potentials. Pharmacol Res. 2015; 95-96: 138-58.
Emadi A, Jones RJ, Brodsky RA. Cyclophosphamide and cancer: Golden anniversary. Nat Rev Clin Oncol. 2009; 6: 638-47.
Fraiser LH, Kanekal S, Kehrer JP. Cyclophosphamide toxicity. Characterising and avoiding the problem. Drugs 1991; 42: 781-95.
Fleer R, Brendel M. Toxicity, interstrand cross-links and DNA fragmentation induced by activated cyclophosphamide in yeast: Comparative studies on 4-hydroperoxycyclophosphamide, its monofunctional analogon, acrolein, phosphoramide mustard, and nornitrogen mustard. Chem Biol Interact. 1982; 39: 1-15.
Guzelsoy P, Aydin S, Basaran N. Potential effects of thymoquinone the active constituent of black seed (Nigella sativa L.) on human health. J Lit Pharm Sci. 2018; 7: 118-35.
Ha H, Yu MR, Choi YJ, Kitamura M, Lee HB. Role of high glucose-induced nuclear factor-kappaB activation in monocyte chemoattractant protein-1 expression by mesangial cells. J Am Soc Nephrol. 2002; 13: 894-902.
Hamzeh M, Hosseinimehr SJ, Mohammadi HR, Yaghubi Beklar S, Dashti A, Talebpour Amiri F. Atorvastatin attenuates the ovarian damage induced by cyclophosphamide in rat: An experimental study. Int J Reprod Biomed. 2018; 16: 323-34.
Khedr NF. Protective effect of mirtazapine and hesperidin on cyclophosphamide-induced oxidative damage and infertility in rat ovaries. Exp Biol Med (Maywood). 2015; 240: 1682-89.
Kisaoglu A, Borekci B, Yapca OE, Bilen H, Suleyman H. Tissue damage and oxidant/antioxidant balance. Eurasian J Med. 2013; 45: 47-49.
Korkmaz A, Topal T, Oter S. Pathophysiological aspects of cyclophosphamide and ifosfamide induced hemorrhagic cystitis: Implication of reactive oxygen and nitrogen species as well as PARP activation. Cell Biol Toxicol. 2007; 23: 303-12.
Liu J, Wang W, Chen L, Li Y, Zhao S, Liang Y. Chemoprotective effect of syringic acid on cyclophosphamide induced ovarian damage via inflammatory pathway. J Oleo Sci. 2021; 70: 675-83.
Malik SW, Myers JL, DeRemee RA, Specks U. Lung toxicity associated with cyclophosphamide use: Two distinct patt-erns. Am J Respir Crit Care Med. 1996; 154: 1851-56.
Melekoglu R, Ciftci O, Eraslan S, Cetin A, Basak N. Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model. J Ovarian Res. 2018; 11: 33.
Mete Ural U, Bayoglu Tekin Y, Sehitoglu I, Kalkan Y, Cumhur Cure M. Biochemical, histopathological and immunohistochemical evaluation of the protective and therapeutic effects of thymoquinone against ischemia and ischemia/reperfusion injury in the rat ovary. Gynecol Obstet Invest. 2016; 81: 47-53.
Mills KA, Chess-Williams R, McDermott C. Novel insights into the mechanism of cyclophosphamide-induced bladder toxi-city: Chloroacetaldehyde's contribution to urothelial dysfunction in vitro. Arch Toxicol. 2019; 93: 3291-303.
Nagi MN, Al-Shabanah OA, Hafez MM, Sayed-Ahmed MM. Thymoquinone supplementation attenuates cyclophosphamide-induced cardiotoxicity in rats. J Biochem Mol Toxicol. 2011; 25: 135-42.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351-58.
Rajasekaran M. Nephroprotective effect of Costus pictus extract against doxorubicin-induced toxicity on Wistar rat. Bangladesh J Pharmacol. 2019; 14: 93-100.
Saleh DO, Mansour DF. Ovario-protective effects of genistein against cyclophosphamide toxicity in rats: Role of anti-mullerian hormone and oestradiol. Eur J Pharmacol. 2016; 789: 163-71
Salman S, Kumbasar S, Yilmaz M, Kumtepe Y, Borekci B, Bakan E, Suleyman H. Investigation of the effects of the chronic administration of some antihypertensive drugs on enzymatic and non-enzymatic oxidant/antioxidant parameters in rat ovarian tissue. Gynecol Endocrinol. 2011; 27: 895-99.
Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968; 25: 192-205.
Singh RJ. Glutathione: A marker and antioxidant for aging. J Lab Clin Med. 2002; 140: 380-81.
Suleyman H, Ozcicek A. Molecular mechanism of ischemia reperfusion injury. Arch Basic Clin Res. 2019; 2: 25-27.
Tsai-Turton M, Luong BT, Tan Y, Luderer U. Cyclophosphamide-induced apoptosis in COV434 human granulosa cells involves oxidative stress and glutathione depletion. Toxicol Sci. 2007; 98: 216-30.
Turkler C, Onat T, Yildirim E, Kaplan S, YAZICI G, Mammadov R. An experimental study on the use of lycopene to prevent infertility due to acute oxidative ovarian damage caused by a single high dose of methotrexate. Adv Clin Exp Med. 2020; 29: 5-11.
Urso ML, Clarkson PM. Oxidative stress, exercise, and antioxidant supplementation. Toxicology 2003; 189: 41-54.
Yener NA, Sinanoglu O, Ilter E, Celik A, Sezgin G, Midi A, Deveci U, Aksungar F. Effects of spirulina on cyclophosphamide-induced ovarian toxicity in rats: Biochemical and histomorphometric evaluation of the ovary. Biochem Res Int. 2013; 2013: 764262.
Zhai J, Zhang F, Gao S, Chen L, Feng G, Yin J, Chen W. Schisandra chinensis extract decreases chloroacetaldehyde production in rats and attenuates cyclophosphamide toxicity in liver, kidney and brain. J Ethnopharmacol. 2018; 210: 223-31.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Cagdas Colluoglu, Serdar Balci, Betul Gundogdu, Taha Abdulkadir Coban, Seval Bulut, Halis Suleyman
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
