In vitro shoot proliferation and plant regeneration of Plumbago indica L. (Ractochita), a rare medicinal shrub of Bangladesh

Authors

  • Fowzia Haque Department of Botany, Jahangirnagar University, Savar, Dhaka-1342
  • AKM Sayeed Hassan Department of Botany, Savar College, Savar, Dhaka-1340
  • Miskat Ara Akhter Jahan Biological Research Division, BCSIR Laboratories, Dhaka-1205
  • Shyamal K Roy Department of Botany, Jahangirnagar University, Savar, Dhaka-1342

DOI:

https://doi.org/10.3329/bjsir.v47i2.11452

Keywords:

Plumbago indica, Medicinal plant, Shoot proliferation, Regeneration, Acclimatization

Abstract

A protocol was established for in vitro shoot proliferation and plant regeneration of a rare medicinal shrub of Bangladesh, Plumbago indica L. (Plumbaginaceae) using shoot tip and nodal explants. Best shoot induction was observed on MS medium supplemented with 0.5 mg/l       BAP, in which 92% of nodal explants responded to produce maximum number (42.8 ± 1.18) of shoots per explants. In vitro raised shoots       rooted on half strength of MS medium with 0.5 mg/l IAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting them in pots containing soil where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 82%.

 

DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11452

 

Bangladesh J. Sci. Ind. Res. 47(2), 197-202, 2012

 

 

Downloads

Download data is not yet available.
Abstract
104
PDF
73

Downloads

Published

2012-07-28

How to Cite

Haque, F., Hassan, A. S., Jahan, M. A. A., & Roy, S. K. (2012). In vitro shoot proliferation and plant regeneration of Plumbago indica L. (Ractochita), a rare medicinal shrub of Bangladesh. Bangladesh Journal of Scientific and Industrial Research, 47(2), 197–202. https://doi.org/10.3329/bjsir.v47i2.11452

Issue

Vol. 47 No. 2 (2012)

Section

Articles

License

Bangladesh Council of Scientific and Industrial Research (BCSIR) holds the copyright to all contents published in Bangladesh Journal of Scientific and Industrial Research (BJSIR). A copyright transfer form should be signed by the author(s) and be returned to BJSIR.

The entire contents of the BJSIR are protected under Bangladesh Council of Scientific and Industrial Research (BCSIR) copyrights.

Creative Commons Licence
BJSIR is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC) Creative Commons Attribution-NonCommercial 4.0 International License which allows others remix, tweak, and build upon the articles non-commercially, and although their new works must also acknowledge and be non-commercial, they don’t have to license their derivative works on the same terms.