In vitro shoot proliferation and plant regeneration of Plumbago indica L. (Ractochita), a rare medicinal shrub of Bangladesh

Authors

  • Fowzia Haque Department of Botany, Jahangirnagar University, Savar, Dhaka-1342
  • AKM Sayeed Hassan Department of Botany, Savar College, Savar, Dhaka-1340
  • Miskat Ara Akhter Jahan Biological Research Division, BCSIR Laboratories, Dhaka-1205
  • Shyamal K Roy Department of Botany, Jahangirnagar University, Savar, Dhaka-1342

DOI:

https://doi.org/10.3329/bjsir.v47i2.11452

Keywords:

Plumbago indica, Medicinal plant, Shoot proliferation, Regeneration, Acclimatization

Abstract

A protocol was established for in vitro shoot proliferation and plant regeneration of a rare medicinal shrub of Bangladesh, Plumbago indica L. (Plumbaginaceae) using shoot tip and nodal explants. Best shoot induction was observed on MS medium supplemented with 0.5 mg/l       BAP, in which 92% of nodal explants responded to produce maximum number (42.8 ± 1.18) of shoots per explants. In vitro raised shoots       rooted on half strength of MS medium with 0.5 mg/l IAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting them in pots containing soil where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 82%.

 

DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11452

 

Bangladesh J. Sci. Ind. Res. 47(2), 197-202, 2012

 

 

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Published

2012-07-28

How to Cite

Haque, F., Hassan, A. S., Jahan, M. A. A., & Roy, S. K. (2012). In vitro shoot proliferation and plant regeneration of Plumbago indica L. (Ractochita), a rare medicinal shrub of Bangladesh. Bangladesh Journal of Scientific and Industrial Research, 47(2), 197–202. https://doi.org/10.3329/bjsir.v47i2.11452

Issue

Vol. 47 No. 2 (2012)

Section

Articles

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