In vitro multiple shoot regeneration from corm bud explant in ghet kachu, typhonium trilobatum schott - a medicinal aroid

Authors

  • KK Paul Institute of Biological Sciences, Rajshahi University, Rajshahi-6205
  • MA Bari Institute of Biological Sciences, Rajshahi University, Rajshahi-6205

DOI:

https://doi.org/10.3329/bjsir.v47i2.11454

Keywords:

Araceae, Ghetkachu, Phytohormone, Regeneration and Typhonium trilobatum

Abstract

An efficient in vitro regeneration protocol was developed in medicinal aroid, Ghetkachu (Typhonium trilobatum Schott) using field grown corm bud explant. Highest percentage (75 %) of direct multiple shoot regeneration obtained in MS media supplemented with 5.0 mgL-1BAP + 1.5mg L-1NAA. Callus formation occur (80 %) in MS media containing 0.5mgL-1BAP + 2.0mgL-1NAA. The appearance of calli was white, creamy white light green in colour and the texture of calli were soft, friable and semi hard and compact. Shoot regeneration (85 %) obtained from calli in MS medium having 5.0mgL-1BAP +1.0mgL-1NAA. The regenerated plantlets were successfully acclimatized with loamy fertile soil and survived cent percentage in natural condition.

 

DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11454

 

Bangladesh J. Sci. Ind. Res. 47(2), 211-216, 2012

 

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Published

2012-07-28

How to Cite

Paul, K., & Bari, M. (2012). In vitro multiple shoot regeneration from corm bud explant in ghet kachu, typhonium trilobatum schott - a medicinal aroid. Bangladesh Journal of Scientific and Industrial Research, 47(2), 211–216. https://doi.org/10.3329/bjsir.v47i2.11454

Issue

Vol. 47 No. 2 (2012)

Section

Articles

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