In vitro multiple shoot regeneration from corm bud explant in ghet kachu, typhonium trilobatum schott - a medicinal aroid

Authors

  • KK Paul Institute of Biological Sciences, Rajshahi University, Rajshahi-6205
  • MA Bari Institute of Biological Sciences, Rajshahi University, Rajshahi-6205

DOI:

https://doi.org/10.3329/bjsir.v47i2.11454

Keywords:

Araceae, Ghetkachu, Phytohormone, Regeneration and Typhonium trilobatum

Abstract

An efficient in vitro regeneration protocol was developed in medicinal aroid, Ghetkachu (Typhonium trilobatum Schott) using field grown corm bud explant. Highest percentage (75 %) of direct multiple shoot regeneration obtained in MS media supplemented with 5.0 mgL-1BAP + 1.5mg L-1NAA. Callus formation occur (80 %) in MS media containing 0.5mgL-1BAP + 2.0mgL-1NAA. The appearance of calli was white, creamy white light green in colour and the texture of calli were soft, friable and semi hard and compact. Shoot regeneration (85 %) obtained from calli in MS medium having 5.0mgL-1BAP +1.0mgL-1NAA. The regenerated plantlets were successfully acclimatized with loamy fertile soil and survived cent percentage in natural condition.

 

DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11454

 

Bangladesh J. Sci. Ind. Res. 47(2), 211-216, 2012

 

Downloads

Download data is not yet available.
Abstract
124
PDF
121

Downloads

Published

2012-07-28

How to Cite

Paul, K., & Bari, M. (2012). In vitro multiple shoot regeneration from corm bud explant in ghet kachu, typhonium trilobatum schott - a medicinal aroid. Bangladesh Journal of Scientific and Industrial Research, 47(2), 211–216. https://doi.org/10.3329/bjsir.v47i2.11454

Issue

Vol. 47 No. 2 (2012)

Section

Articles

License

Bangladesh Council of Scientific and Industrial Research (BCSIR) holds the copyright to all contents published in Bangladesh Journal of Scientific and Industrial Research (BJSIR). A copyright transfer form should be signed by the author(s) and be returned to BJSIR.

The entire contents of the BJSIR are protected under Bangladesh Council of Scientific and Industrial Research (BCSIR) copyrights.

Creative Commons Licence
BJSIR is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC) Creative Commons Attribution-NonCommercial 4.0 International License which allows others remix, tweak, and build upon the articles non-commercially, and although their new works must also acknowledge and be non-commercial, they don’t have to license their derivative works on the same terms.