Isolation, Characterization of <i>Neurospora crassa</i> Mutants Exhibiting Growth Inhibition and Mutagenesis of this Fungus by the Leaf Extracts of <i>Leucas aspera</i> (Willd) Link

Authors

  • M Nazrul Islam Bhuiyan BCSIR Laboratories, Chittagong-4220, Bangladesh
  • TIMA Mozmader Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh
  • M Amirul Hoque BCSIR Laboratories, Dhaka-1205, Bangladesh
  • Sumi Akter ICDDRB, Dhaka, Bangladesh
  • Jahedul Islam Mahmud BCSIR Laboratories, Chittagong-4220, Bangladesh

DOI:

https://doi.org/10.3329/bjsir.v43i3.1155

Keywords:

Neurospora crassa, Leucas aspera, Mutants, Dirty, Vigorous, Cauliflower and Conidial brand.

Abstract

Ten Neurospora crassa mutants obtained by chemical mutagenesis were screened in Leucas aspera (willd) Link. (Bengali name- swetadrone) plants. The aqueous extracts of the leaves of L. aspera showed significant inhibition of growth and mutagenic effect on N. crassa. Results showed that 1 ml extract reacted with the test organism of N. crassa very rapidly. During the period of 24 hours, it produced only 2.6 cm linear vegetative growth of the mycelia. Whereas, 1 ml and 0.50 ml extracts exhibited mild reactions (3.20 and 3.90 cm. respectively). The linear growth decreased with the increase of the concentrations of extracts. Conidia of Ema (5297) of N. crassa were treated for 3-4 hours in 100% concentration of the extracts. 100% extracts produced ten types of mutants, namely- dirty, vigorus, ropy, albino, mat, plug, check, fluppy, cauliflower and conidial brand.

Key words: Neurospora crassa, Leucas aspera, Mutants, Dirty, Vigorous, Cauliflower and Conidial brand.  

DOI = 10.3329/bjsir.v43i3.1155

Bangladesh J. Sci. Ind. Res. 43(3), 387-396, 2008

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How to Cite

Bhuiyan, M. N. I., Mozmader, T., Hoque, M. A., Akter, S., & Mahmud, J. I. (2008). Isolation, Characterization of <i>Neurospora crassa</i> Mutants Exhibiting Growth Inhibition and Mutagenesis of this Fungus by the Leaf Extracts of <i>Leucas aspera</i> (Willd) Link. Bangladesh Journal of Scientific and Industrial Research, 43(3), 387–396. https://doi.org/10.3329/bjsir.v43i3.1155

Issue

Vol. 43 No. 3 (2008)

Section

Articles

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