In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis

Authors

  • AKMS Hassan Department of Botany, Savar College, Savar, Dhaka
  • F Haque Biological Research Division, BCSIR Laboratories, Dhaka
  • MAA Jahan Biological Research Division, BCSIR Laboratories, Dhaka
  • SK Roy Department of Botany, Jahangirnagar University, Savar, Dhaka

DOI:

https://doi.org/10.3329/bjsir.v47i3.13063

Keywords:

Plumbago zeylanica, Medicinal plant, Organogenesis, Shoot proliferation, Mass propagation, Acclimatization

Abstract

An efficient protocol was developed for in vitro mass propagation of an important medicinal shrub, Plumbago zeylanica L., (Plumbaginaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP, in which 86.4% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IAA. For acclimatization and transplantation, the plantlets in the rooted culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%.

DOI: http://dx.doi.org/10.3329/bjsir.v47i3.13063

Bangladesh J. Sci. Ind. Res. 47(3), 297-302 2012

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Published

2012-12-21

How to Cite

Hassan, A., Haque, F., Jahan, M., & Roy, S. (2012). In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis. Bangladesh Journal of Scientific and Industrial Research, 47(3), 297–302. https://doi.org/10.3329/bjsir.v47i3.13063

Issue

Vol. 47 No. 3 (2012)

Section

Articles

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