Callus induction and plant regeneration of paederia foetida L., a widely used medicinal vine in Bangladesh

Authors

  • AKMS Hassan Department of Botany, Savar College, Savar, Dhaka-1340,
  • CK Roy Biological Research Division, BCSIR Laboratories, Dhaka-1205,
  • R Sultana Biological Research Division, BCSIR Laboratories, Dhaka-1205,
  • R Khatun Biological Research Division, BCSIR Laboratories, Dhaka-1205,

DOI:

https://doi.org/10.3329/bjsir.v47i4.14066

Keywords:

Paederia foetida, Medicinal plant, Callus culture, Shoot proliferation, Regeneration, Acclimatization

Abstract

An efficient protocol was established for in vitro plant regeneration of Paederia foetada L. (Family. Rubiaceae), a widely used medicinal vine of Bangladesh through callus culture in using nodal segment. Yellowish green nodular callus was observed from nodal segments on MS basal medium supplemented with 1.5 mg/L BAP + 0.5 mg/L NAA within three weeks. Large number of shoots (14.4±1.29) were obtained when the callus was sub cultured on MS medium with 0.5 mg/L BAP. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/L IBA. The survival rate of plantlets was found to be 85%. Regenerated plants were morphologically uniform having normal leaf shape and growth.

Bangladesh J. Sci. Ind. Res. 47(4), 373-378, 2012

DOI: http://dx.doi.org/10.3329/bjsir.v47i4.14066

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Published

2013-03-06

How to Cite

Hassan, A., Roy, C., Sultana, R., & Khatun, R. (2013). Callus induction and plant regeneration of paederia foetida L., a widely used medicinal vine in Bangladesh. Bangladesh Journal of Scientific and Industrial Research, 47(4), 373–378. https://doi.org/10.3329/bjsir.v47i4.14066

Issue

Vol. 47 No. 4 (2012)

Section

Articles

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