In vitro regeneration and molecular characterization of some varieties of Lycopersicon esculentum Mill. in Bangladesh

  • M Billah Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh
  • TA Banu Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh
  • M Islam Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh
  • NA Banu Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh
  • S Khan Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh
  • S Akter Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh
  • A Habib Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh
Keywords: Lycopersicon esculentum; Leaf explants; Multiple shoot; RAPD analysis

Abstract

An efficient regeneration protocol was established for two varieties (BARI tomato-9 and BARI tomato-15) of tomato (Lycopersicon esculentum Mill.) using three explants namely cotyledonary node, cotyledonary leaf and hypocotyls. Among the three explants, maximum number of shoots was produced from cotyledonary leaf explants of BARI tomato-15 on MS with 2.0 mg/l BAP and 0.5 mg/l IAA. In this combination of BAP and IAA 86%, on an average, cotyledonary leaf explants showed regeneration response 14.12 shoots/explants. Explants from hypocotyl showed best results in MS medium with 2.0 mg/l BAP and 0.2 mg/l IAA in both the varieties. In case of cotyledonary node, BARI tomato-15 showed 6.0 shoot/explant on MS with 2.0 mg/l BAP and 1.0 mg/l IAA. Molecular characterization of total ten varieties of tomato in Bangladesh was done by using six arbitrary oligonucleotide RAPD primers. A total of 140 bands were produced where the highest genetic distance (0.6769) was found between BARI tomato-3 and Mintoo tomato and lowest distance (0.1035) was observed between BARI tomato-7 and BARI tomato-8. This result will be useful for designing future breeding programs.

Bangladesh J. Sci. Ind. Res.54(2), 117-124, 2019

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Published
2019-06-01
How to Cite
Billah, M., Banu, T., Islam, M., Banu, N., Khan, S., Akter, S., & Habib, A. (2019). In vitro regeneration and molecular characterization of some varieties of Lycopersicon esculentum Mill. in Bangladesh. Bangladesh Journal of Scientific and Industrial Research, 54(2), 117-124. https://doi.org/10.3329/bjsir.v54i2.41667
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