Peroxidase from infected fruit of Solanum sp. grown in Nsukka

Authors

  • Omeje KO Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
  • Eze SOO Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
  • FC Chilaka Department of Biochemistry, University of Nigeria, Nsukka, Nigeria

DOI:

https://doi.org/10.3329/bjsir.v54i2.41669

Keywords:

Enzyme; Characterization; Infected fruit; Solanum spp; Peroxidase

Abstract

In this study, we characterized the activity of peroxidase a quality control enzyme from the infected fruit of Solanum sp. Peroxidase was purified to homogeneity by ammonium sulfate precipitation, dialysis, ion exchange chromatography and size exclusion chromatography. The molecular weight of the native enzyme was 63000 da. The enzyme was shown to have two iso-enzymes with distinct optimum pH of 4.5 and 7.0 and optimum temperature of 40 and 70⁰C. The purified enzyme had broad substrate specificity with o-dianisidine being the ideal substrate. Na+, Ca2+, Mg2+, Mn2+, Cu2+, Al3+ were shown to be activators of the enzyme, while the peroxidase activity was severely inhibited by Co2+.

Bangladesh J. Sci. Ind. Res.54(2), 131-138, 2019

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Published

2019-06-01

How to Cite

KO, O., SOO, E., & Chilaka, F. (2019). Peroxidase from infected fruit of Solanum sp. grown in Nsukka. Bangladesh Journal of Scientific and Industrial Research, 54(2), 131–138. https://doi.org/10.3329/bjsir.v54i2.41669

Issue

Vol. 54 No. 2 (2019)

Section

Articles

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