<i>In Vitro</i> Mass Propagation of <i>Heliotropium indicum</i> L., using Apical and Axillary Bud Explants

Authors

  • AKM Sayeed Hassan Biological Research Division, BCSIR Laboratories, Dhaka
  • Nadira Begum Biological Research Division, BCSIR Laboratories, Dhaka
  • Miskat Ara Akhter Jahan Biological Research Division, BCSIR Laboratories, Dhaka
  • Rahima Khatun Biological Research Division, BCSIR Laboratories, Dhaka

DOI:

https://doi.org/10.3329/bjsir.v45i1.5185

Keywords:

Heliotropium indicum, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization

Abstract

A consequency was obtained for mass propagation of a valuable ayurvedic medicinal herb, Heliotropium indicum Linn. (Boraginaceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l GA3, in which 92% of the axillary buds explants produced 12 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 18 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%.

Key words: Heliotropium indicum; Medicinal plant; Shoot proliferation; Micropropagation; Acclimatization.

DOI: 10.3329/bjsir.v45i1.5185

Bangladesh J. Sci. Ind. Res. 45(1), 69-74, 2010

Downloads

Download data is not yet available.
Abstract
139
PDF
131

Downloads

How to Cite

Hassan, A. S., Begum, N., Jahan, M. A. A., & Khatun, R. (2010). <i>In Vitro</i> Mass Propagation of <i>Heliotropium indicum</i> L., using Apical and Axillary Bud Explants. Bangladesh Journal of Scientific and Industrial Research, 45(1), 69–74. https://doi.org/10.3329/bjsir.v45i1.5185

Issue

Vol. 45 No. 1 (2010)

Section

Articles

License

Bangladesh Council of Scientific and Industrial Research (BCSIR) holds the copyright to all contents published in Bangladesh Journal of Scientific and Industrial Research (BJSIR). A copyright transfer form should be signed by the author(s) and be returned to BJSIR.

The entire contents of the BJSIR are protected under Bangladesh Council of Scientific and Industrial Research (BCSIR) copyrights.

Creative Commons Licence
BJSIR is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC) Creative Commons Attribution-NonCommercial 4.0 International License which allows others remix, tweak, and build upon the articles non-commercially, and although their new works must also acknowledge and be non-commercial, they don’t have to license their derivative works on the same terms.