In Vitro plant regeneration from leaf explants of Tagetes erecta L.

Authors

  • JL Munshi Biological Research Division, Bangladesh Council of Scientific & Industrial Research (BCSIR), Dhaka-1205, Bangladesh
  • R Baksha Biological Research Division, Bangladesh Council of Scientific & Industrial Research (BCSIR), Dhaka-1205, Bangladesh
  • MZ Rahaman Biological Research Division, Bangladesh Council of Scientific & Industrial Research (BCSIR), Dhaka-1205, Bangladesh
  • NN Huque Biological Research Division, Bangladesh Council of Scientific & Industrial Research (BCSIR), Dhaka-1205, Bangladesh
  • EA Zinat Biological Research Division, Bangladesh Council of Scientific & Industrial Research (BCSIR), Dhaka-1205, Bangladesh
  • N Momtaz Biological Research Division, Bangladesh Council of Scientific & Industrial Research (BCSIR), Dhaka-1205, Bangladesh

DOI:

https://doi.org/10.3329/bjsir.v56i2.54313

Keywords:

In vitro; Tagetes erecta L.; Callus induction; Regeneration; Acclimatization

Abstract

Regeneration of multiple shoots via callus induction and organogenesis was obtained from young leaf explants of the field grown marigold (Tagetes erecta L.). Callus induction and shoot regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. Highest percentage (90%) of callus formation was observed within two weeks on MS medium supplemented with 5.0 mg/l BAP with 2.5 mg/l NAA. The maximum percentage (80%) of shoot bud formation (10±0.5/callus) was obtained from MS medium containing 1.0 mg/l BAP with 0.5 mg/l kinetin. The regenerated shoots developed highest percentages (90%) of roots on half strength MS medium supplemented with 1.0 mg/l IBA. The plantlets when transferred into potsoil 80% survived. Regenerated plants were morphologically uniform with normal leaf shape and growth pattern.

Bangladesh J. Sci. Ind. Res.56(2), 69-74, 2021

Downloads

Download data is not yet available.
Abstract
52
PDF
42

Downloads

Published

2021-06-23

How to Cite

Munshi, J., Baksha, R., Rahaman, M., Huque, N., Zinat, E., & Momtaz, N. (2021). In Vitro plant regeneration from leaf explants of Tagetes erecta L. Bangladesh Journal of Scientific and Industrial Research, 56(2), 69–74. https://doi.org/10.3329/bjsir.v56i2.54313

Issue

Section

Articles