<i>In vitro</i> Plant Regeneration from Axillary Buds of <i>Asparagus racemosus</i> Wild, a Medicinal Plant
AbstractAn efficient method was developed for regeneration of Asparagus racemosus Wild, from axillary explants. MS media supplemented with different concentrations of cytokinin (BAP) alone or in combination of different concentrations of auxin (NAA or IBA) was tested for their efficiency in multiple shoot induction. High frequency of multiple shoot regeneration was achieved on MS medium supplemented with BAP (0.1mg/l) and NAA (0.05 mg/l). When shoots were well developed they were dissected and subcultured on the same medium to promote more multiple shoots. Eighteen to twenty shoots were obtained on this medium. The shoots were rooted best on half MS medium supplemented with 0.05 mg/l BAP and 1.0 mg/l IBA. Among the five levels of pH tested, 5.7 was the best for multiple shoot proliferation. The result presented here proved to be suitable for the rapid propagation system of A. racemosus.
Key words: Asparagus racemosus; Medicinal plant; Rapid proliferation; Multiple shoots; Auxins and Cytokinins
Bangladesh J. Sci. Ind. Res. 45(3), 255-260, 2010
Bangladesh Council of Scientific and Industrial Research (BCSIR) holds the copyright to all contents published in Bangladesh Journal of Scientific and Industrial Research (BJSIR). A copyright transfer form should be signed by the author(s) and be returned to BJSIR.
The entire contents of the BJSIR are protected under Bangladesh Council of Scientific and Industrial Research (BCSIR) copyrights.
BJSIR is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC) Creative Commons Attribution-NonCommercial 4.0 International License which allows others remix, tweak, and build upon the articles non-commercially, and although their new works must also acknowledge and be non-commercial, they dont have to license their derivative works on the same terms.