<i>In Vitro</i> Shoot Proliferation and Plant Regeneration of <i>Phyllanthus fraternus</i> Webster (B. Bhuiamla), a Seasonal Medicinal Herb

Authors

  • AKM Sayeed Hassan Department of Botany, Savar College, Savar, Dhaka
  • R Khatun Biological Research Division, BCSIR Laboratories, Dhaka

DOI:

https://doi.org/10.3329/bjsir.v46i2.8187

Keywords:

Phyllanthus fraternus, Medicinal plant, Shoot proliferation, Regeneration, Acclimatization

Abstract

An efficient system was developed for shoot proliferation and large scale plant regeneration of a seasonal multipotent medicinal herb, Phyllanthus fraternus Webster through in vitro culture. Shoot tips and nodal explants of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l GA3, in which 88% of nodal explants responded to produce maximum number (16.8 ± 0.95) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 82%.

Key words: Phyllanthus fraternus; Medicinal plant; Shoot proliferation; Regeneration; Acclimatization

DOI: http://dx.doi.org/10.3329/bjsir.v46i2.8187

Bangladesh J. Sci. Ind. Res. 46(2), 205-210, 2011

Downloads

Download data is not yet available.
Abstract
136
PDF
95

Downloads

How to Cite

Hassan, A. S., & Khatun, R. (2011). <i>In Vitro</i> Shoot Proliferation and Plant Regeneration of <i>Phyllanthus fraternus</i> Webster (B. Bhuiamla), a Seasonal Medicinal Herb. Bangladesh Journal of Scientific and Industrial Research, 46(2), 205–210. https://doi.org/10.3329/bjsir.v46i2.8187

Issue

Vol. 46 No. 2 (2011)

Section

Articles

License

Bangladesh Council of Scientific and Industrial Research (BCSIR) holds the copyright to all contents published in Bangladesh Journal of Scientific and Industrial Research (BJSIR). A copyright transfer form should be signed by the author(s) and be returned to BJSIR.

The entire contents of the BJSIR are protected under Bangladesh Council of Scientific and Industrial Research (BCSIR) copyrights.

Creative Commons Licence
BJSIR is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC) Creative Commons Attribution-NonCommercial 4.0 International License which allows others remix, tweak, and build upon the articles non-commercially, and although their new works must also acknowledge and be non-commercial, they don’t have to license their derivative works on the same terms.