<i>In Vitro</i> Plant Regeneration from Cultured Cotyledons of Cotton (<i>Gossypium herbaceum<i> L.)
The effect of cytokinins on callus proliferation from cotyledons and plantlet development was studied in cotton. The frequency of callus induction was observed on MS medium enriched with a variety of cytokinins in different concentrations. With the increase of cytokinin concentration, the percentage of callus formation, percentage of shoot developing calli and number of shoots/calli were increased. Among the three different cytokinins studied, BA showed the highest performance. The highest percentage of callus (6.55%) and shoot developing calli (5.87%) was obtained on MS with 1.0 mg/l BA. Highest number of shoots (3.02) per calli was observed on MS media supplemented with 1.0 mg/l Kn. The rooting media composed of MS medium, 0.6% agar, sucrose and fortified with 2.0 mg/l NAA induced root development at the highest percentage (41.23%) with maximum number of roots (3.61) per cutting and length of root (3.62 cm) per culture. The plantlets were acclimatized in natural conditions.
Key words: In vitro; Callus; Cotyledons; Cytokinin; Plantlet; Acclimatization
BJSIR 2011; 46(3): 359-364
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