High Frequency <i>In Vitro</i> Regeneration of <i>Dianthus caryophyllus</i> L., a Herbaceous Perennial Ornamental Plant

Authors

  • AKM Sayeed Hassan Biological Research Division, BCSIR Laboratories, Dhaka
  • JL Munshi Biological Research Division, BCSIR Laboratories, Dhaka
  • R Sultana Biological Research Division, BCSIR Laboratories, Dhaka
  • MAA Jahan Biological Research Division, BCSIR Laboratories, Dhaka
  • R Khatun Biological Research Division, BCSIR Laboratories, Dhaka

DOI:

https://doi.org/10.3329/bjsir.v46i4.9597

Keywords:

Dianthus caryophyllus, Shoot proliferation, Micropropagation, Acclimatization

Abstract

High frequency plant regeneration was established from shoot tips and nodal explants of a perennial ornamental plant, Dianthus caryophyllus L. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l NAA, in which 82% of the explants responded to produce maximum number of shoots (38) per culture. In vitro raised healthy shoots were rooted on half strength MS medium with 0.5 mg/l IBA +0.5 mg/l NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 78%.

Key words: Dianthus caryophyllus; Shoot proliferation; Micropropagation; Acclimatization

DOI: http://dx.doi.org/10.3329/bjsir.v46i4.9597

BJSIR 2011; 46(4): 495-498

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How to Cite

Hassan, A. S., Munshi, J., Sultana, R., Jahan, M., & Khatun, R. (2012). High Frequency <i>In Vitro</i> Regeneration of <i>Dianthus caryophyllus</i> L., a Herbaceous Perennial Ornamental Plant. Bangladesh Journal of Scientific and Industrial Research, 46(4), 495–498. https://doi.org/10.3329/bjsir.v46i4.9597

Issue

Vol. 46 No. 4 (2011)

Section

Articles

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