Micropropagation of <i>Eclipta alba</i> (Linn.) Hassk- a Valuable Medicinal Herb

Authors

  • AKM Sayeed Hassan Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh
  • Farhana Afroz Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh
  • Laila Shamroze Bari Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh
  • John Liton Munshi Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh
  • Miskat Ara Akhter Jahan Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh
  • Rahima Khatun Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh

DOI:

https://doi.org/10.3329/bjsir.v43i2.965

Keywords:

Eclipta alba, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization

Abstract

A protocol was established for mass propagation of a valuable medicinal herb, Eclipta alba (Linn.) Hassk (Asteraceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mgl-1 BAP + 0.1 mgl-1 NAA, in which 94% of the explants produced 18 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 26 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mgl-1 IBA +1.0 mgl-1 NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%.

Key words: Eclipta alba, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization   

DOI: 10.3329/bjsir.v43i2.965

Bangladesh J. Sci. Ind. Res. 43(2), 215-222, 2008

 

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How to Cite

Hassan, A. S., Afroz, F., Bari, L. S., Munshi, J. L., Jahan, M. A. A., & Khatun, R. (2008). Micropropagation of <i>Eclipta alba</i> (Linn.) Hassk- a Valuable Medicinal Herb. Bangladesh Journal of Scientific and Industrial Research, 43(2), 215–222. https://doi.org/10.3329/bjsir.v43i2.965

Issue

Vol. 43 No. 2 (2008)

Section

Articles

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