Gamma interferon levels among Bangladeshi children after measles vaccination

Authors

  • S Sultana Department of Virology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka
  • S Tabassum Department of Virology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka
  • A Nessa Department of Virology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka
  • M Jahan Department of Virology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka

DOI:

https://doi.org/10.3329/bmrcb.v40i3.25234

Abstract

Gamma interferon (IFN-?) plays an important role in the immune response to live measles virus vaccination. To study the immune response to measles vaccination, IFN-? level was estimated in 30 children. Of these, 24 children vaccinated with a single dose of measles vaccine at nine months of age and 06 children vaccinated with a second dose during the Measles Catch-up Immunization campaign. Measles vaccine strain was cultured in Vero cell line and the Tissue Culture Infective Dose (TCID)50 was used as standard live virus. Peripheral blood Mononuclear cells (PBMCs) was separated by Ficoll- Hypaque density gradient centrifugation and stimulated with measles virus antigens and mitogens (lectin), cultured in CO2 and IFN-? level was measured from culture supernatant by ELISA. On stimulation with measles antigen and lectin respectively, IFN-? level was highest (105 pg/ml and 226.54 pg/ml) in the 109-120 months age group while it was lowest (12.97±8.16 pg/ml and 13.16±8.0 pg/ml) in the 61-72 months age group. No significant difference was observed in IFN-? level after stimulation with either measles antigen or lectin among well-nourished (p<0.8) and mal-nourished (p<0.7) children suggesting that nutritional status did not have any effect on IFN-? level. However, IFN-? level was higher in children who received two dose of measles vaccine than those who received a single dose (p<0.001).

Bangladesh Med Res Counc Bull 2014; 40 (3): 118-121

Downloads

Download data is not yet available.
Abstract
170
PDF
134

Author Biography

S Sultana, Department of Virology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka



Downloads

Published

2015-10-05

How to Cite

Sultana, S., Tabassum, S., Nessa, A., & Jahan, M. (2015). Gamma interferon levels among Bangladeshi children after measles vaccination. Bangladesh Medical Research Council Bulletin, 40(3), 118–121. https://doi.org/10.3329/bmrcb.v40i3.25234

Issue

Vol. 40 No. 3 (2014)

Section

Research Papers

License

Authors who publish with this journal agree to the following terms.

Authors retain copyright and grant the journal right of first publication.

Creative Commons Licence
Articles in the Bangladesh Medical Research Council Bulletin are licensed under a Creative Commons Attribution 4.0 International License (CC-BY) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).