Enhanced detection rate of typhoid fever among clinically suspected patients in a tertiary referral hospital in Dhaka, Bangladesh using nested polymerase chain reaction technology

Authors

  • Shafinaz Khan Dr. Sirajul Islam Medical College, Mougbazar, Dhaka
  • Ruhul Amin Mia Department of Microbiology, Bangabandhu Sheikh Mujib Medical College, Dhaka
  • Sumaiya Khatun Department of Microbiology, City Medical College, Gazipur.

DOI:

https://doi.org/10.3329/bmrcb.v41i3.29971

Keywords:

Typhoid fever, Nested polymerase chain reaction, Diagnosis

Abstract

A nested polymerase chain reaction (PCR) specific for Salmonella enterica subspecies enteric serovar Typhi was used for the detection of the pathogen in blood. This study was done during the period of March 2013 to February 2014. A total of 80 clinically suspected cases of typhoid fever were included in the study. Blood was collected from all participating individuals. Nested PCR targeting the flagellin gene (fliC) of Salmonella Typhi & blood culture were done for each of the cases. The positivity rate of PCR & blood culture was 70%& 20% respectively. The positivity rate of PCR was significantly higher than blood culture (P< 0.05). With the nested PCR, S. Typhi DNAs were detected from blood specimens of 67.2% (43/64) patients among the suspected typhoid fever cases on the basis of clinical features but with negative cultures. We conclude that the PCR technique could be used as a novel diagnostic method of typhoid fever, particularly in culture-negative cases in an endemic country like Bangladesh.

Downloads

Download data is not yet available.
Abstract
49
PDF
50

Downloads

Published

2016-10-17

How to Cite

Khan, S., Mia, R. A., & Khatun, S. (2016). Enhanced detection rate of typhoid fever among clinically suspected patients in a tertiary referral hospital in Dhaka, Bangladesh using nested polymerase chain reaction technology. Bangladesh Medical Research Council Bulletin, 41(3), 138–143. https://doi.org/10.3329/bmrcb.v41i3.29971

Issue

Vol. 41 No. 3 (2015)

Section

Research Papers

License

Authors who publish with this journal agree to the following terms.

Authors retain copyright and grant the journal right of first publication.

Creative Commons Licence
Articles in the Bangladesh Medical Research Council Bulletin are licensed under a Creative Commons Attribution 4.0 International License (CC-BY) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).