Islet cell and glutamic acid decarboxylase-65 autoantibodies in young diabetic patients attending in a General Hospital in Dhaka City

Autoantibodies in young diabetic patients

Authors

  • Ashesh Kumar Chowdhury Department of Immunology, BIRDEM General Hospital, Dhaka, Bangladesh. http://orcid.org/0000-0002-7112-8373
  • Shahjalalur Rahman Sahi Department of Oncology, Khawja Eunus Ali Medical College, Sirajgonj, Bangladesh.
  • Mohammad Moniruzzaman Department of Immunology, Bangladesh University of Health Science, Dhaka, Bangladesh.
  • Mansura Khan Department of Immunology, BIRDEM General Hospital, Dhaka, Bangladesh.

DOI:

https://doi.org/10.3329/bmrcb.v46i2.49019

Keywords:

Islet cell autoantibody, Glutamic acid decarboxylase-65, Latent autoimmune diabetes in youth, Type-2 diabetes

Abstract

Background: Immune mediated destruction of pancreatic beta cell in type-I diabetes is well established but its’ role in young type-2 diabetic patients is still not conclusive. These young diabetic patients pass through several stages where they do not need insulin but found to have serum autoantibody against islets cell and even become dependent on insulin for survival in course of time. This study aims to find the presence of islets cell auto-antibodies (ICA) and autoantibody to glutamic acid decarboxylase-65 (GAD-65) in non-insulin requiring young diabetic patients of Bangladesh.

Objective: To evaluate the presence of ICA and GAD-65 between the non-insulin requiring young type-2 diabetic patients and compare with the non-diabetic control group.

Method: This case control study was carried out at the Department of Immunology, BIRDEM General Hospital, Dhaka for a period of one year from July 2013, A total of 120 non-insulin requiring (≥12 months) young type-2 diabetic patients and 60 age, sex matched non-diabetic were enrolled as control subjects following inclusion and exclusion criteria. ICA and GAD-65 tests were performed by enzyme linked immune-sorbent assay (ELISA) method by using kits from DRG Inc. International, USA.

Results: In this study statistically significant difference found between non insulin requiring young diabetic patients and non diabetic control in respect of positive ICA result (p=0.015). The moderately strong negative association was found between different age of onset of diabetes mellitus and value of ICA level (r=-0.45). Only 20-24 years age group showed statistically significant difference between patient and control (p=0.013). Statistically significant difference was not found in GAD-65 values of non insulin requiring young diabetic patients and non diabetic controls (p=0.441).

Conclusion: This study revealed that there is significant difference present in respect of ICA among non-insulin requiring young diabetic patients and non-diabetic controls. Therefore, autoimmune pathogenesis of beta cell killing by producing ICA against islets cell take place in young type-2 diabetic patients.

Bangladesh Med Res Counc Bull 2020; 46(2): 104-108

Downloads

Download data is not yet available.
Abstract
469
PDF
443 Online view
0

Downloads

Published

2020-08-01

How to Cite

Chowdhury, A. K., Sahi, S. R., Moniruzzaman, M., & Khan, M. (2020). Islet cell and glutamic acid decarboxylase-65 autoantibodies in young diabetic patients attending in a General Hospital in Dhaka City: Autoantibodies in young diabetic patients. Bangladesh Medical Research Council Bulletin, 46(2), 104–108. https://doi.org/10.3329/bmrcb.v46i2.49019

Issue

Vol. 46 No. 2 (2020)

Section

Research Papers

License

Authors who publish with this journal agree to the following terms.

Authors retain copyright and grant the journal right of first publication.

Creative Commons Licence
Articles in the Bangladesh Medical Research Council Bulletin are licensed under a Creative Commons Attribution 4.0 International License (CC-BY) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).