Role of Non-Coding Variants of NLGN-3 and 4x Genes along with Non-Genetic Factors in Autism Spectrum Disorder (ASD) in Selected Population in Bangladesh

Non-coding variants in ASD

Authors

  • Laila Khaleda Department of Genetic Engineering and Biotechonology, University of Chittagong, Bangladesh.
  • Israt Akter Priya Department of Genetic Engineering and Biotechonology, University of Chittagong, Bangladesh.
  • Md. Abdur Rahman Apu Department of Genetic Engineering and Biotechonology, University of Chittagong, Bangladesh.
  • Amit Datta Department of Genetic Engineering and Biotechonology, University of Chittagong, Bangladesh.
  • Basana Rani Muhuri Department of Pediatric Nephrology, Chittagong Medical College Hospital, Chattogram, Bangladesh.
  • Mohammad Al Forkan Department of Genetic Engineering and Biotechonology, University of Chittagong, Bangladesh.

DOI:

https://doi.org/10.3329/bmrcb.v47i3.59237

Keywords:

Autism spectrum disorder, NLGN gene, Genetic abnormalities, Birth complications

Abstract

Background: Autism Spectrum Disorder (ASD) is a group of neurodevelopmental disorder which is now a hotbed worldwide. Being located on chromosome X and playing a vital role in synaptic transmission, NLGN-3 and 4X have been drawn the attention of many researchers as the most essential and functional candidate genes for ASD pathogenesis. However, there are many contradictory results considering their role in developing ASD in different populations.
Objective: This study was aimed to investigate the association of NLGN-3 and 4X genes along with non-genetic factors to develop ASD in Bangladeshi population.
Methods: In this study, we analysed rs4844285 and rs11795613 of NLGN-3 gene and rs3810686 and rs6638575 of NLGN-4X gene for both family-based association analysis and case-control based association analysis using polymerase chain reaction (PCR) and DNA sequencing. Along with this, demographic data were also analysed. A total of 60 members of 15 families, including ASD subjects and another 60 ASD subjects and 60 healthy people were included in this study.
Results: Allele A and genotype AA of NLGN-3 rs4844285 were found to be a probable risk factor for developing ASD in our studied population (p=0.031 for allele A, S x2 = 2.707, OR=1.833 for genotype AA). We also found different birth complications in this studied population which can be considered to intensify the risk of ASD along with the abnormalities of genes.
Conclusion: From this study, it is clear that not only genetic abnormalities but also some birth anomalies play a vital role for the risk of developing ASD.

Bangladesh Med Res Counc Bull 2021; 47(3): 250-259

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Published

2022-07-28

How to Cite

Khaleda, L., Priya, I. A., Apu, M. A. R., Datta, A., Muhuri, B. R., & Al Forkan, M. (2022). Role of Non-Coding Variants of NLGN-3 and 4x Genes along with Non-Genetic Factors in Autism Spectrum Disorder (ASD) in Selected Population in Bangladesh: Non-coding variants in ASD. Bangladesh Medical Research Council Bulletin, 47(3), 250–259. https://doi.org/10.3329/bmrcb.v47i3.59237

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Section

Research Papers