Evaluation of plasma microRNA 122 levels among different groups of chronic hepatitis B patients
plasma microRNA level in chronic hepatitis B
Keywords:Micro RNA 122, Real time PCR, Alphafeto protein, HBV DNA
Background: MicroRNA-122 (miR-122) is the most abundant liver-specific microRNA in humans which is released into the blood circulation in significant amounts from injured liver tissues. This circulating miRNA-122 can differentiate between chronic HBV carriers with high and low risks of disease progression.
Objective: To estimate the plasma levels of microRNA-122 in different groups of chronic hepatitis B (CHB) patients in Bangladesh.
Methods: The study consisted of 70 participants which included 20 CHB patients with hepatocellular carcinoma (HCC), 20 CHB patients with cirrhosis, 20 CHB patients without HCC and cirrhosis, along with 10 healthy controls (HC). Total RNA was extracted from plasma samples followed by cDNA synthesis and finally level of miR-122 was analysed using real time PCR technique.
Results: Level of miR-122 in plasma samples was elevated considerably in all patient groups under study compared to the healthy controls. It was 63.28±18.52 (mean ±SEM), 32.8±7.37 (mean ±SEM), and 125.84±24.8 (mean ±SEM) folds higher in CHB group with HCC, CHB group with cirrhosis and CHB group without HCC and cirrhosis respectively than that in the healthy control group. Furthermore, the plasma miR-122 level correlated positively with HBV DNA viral load in all groups of CHB patients but had no significant correlation with serum ALT and AFP levels. The receiver-operator characteristic (ROC) curves of plasma levels of miR-122 were generated to determine the specificity and sensitivity of this micro RNA to distinguish the patient groups from healthy controls. The plasma miR-122 showed sensitivity and specificity of 95% and 90%, 85% and 90%, 100% and 90% respectively when differentiating CHB group with HCC, CHB group with cirrhosis and CHB group without HCC and cirrhosis from healthy controls.
Conclusion: It may be concluded that, plasma level of miR-122 was increased in all the patient groups with CHB infection and correlated positively with HBV DNA viral load.
Bangladesh Med Res Counc Bull 2021; 47(3): 302-307
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Copyright (c) 2022 Tania Islam Resma, Munira Jahan, Raad Rahmat, Shahina Tabassum
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