Usefulness of Light Emitting Diode (LED) fluorescent microscopy as a tool for rapid and effective method for the diagnosis of pulmonary tuberculosis

Authors

  • Z Khatun DGHS, Dhaka
  • M Kamal NTRL, NIDCH, Dhaka
  • CK Roy Department of Clinical Pathology, BSMMU, Dhaka
  • T Sultana Department of Clinical Pathology, BSMMU, Dhaka,
  • MQ Rahman Department of Clinical Pathology, BSMMU, Dhaka,
  • MBAS Azad Narsingdi Zilla Hospital, Narsingdi
  • ANN Ahmed Department of Clinical Pathology, BSMMU, Dhaka

DOI:

https://doi.org/10.3329/bmrcb.v37i1.7792

Keywords:

LED, pulmonary tuberculosis

Abstract

Background: Tuberculosis remains world's leading cause of death from a single infectious agent. Fluorescence microscopy offers well-described benefits, comparing with brightfield microscopy, for the evaluation sputum smear samples for tuberculosis. We evaluated the diagnostic performance of fluorescence microscopy, using novel Light Emitting Diode (LED) technology as an alternative to the conventional fluorescence microscopy by Auramine stain as well as brightfield microscopy by Ziehl- Neelsen (ZN) stain.

Objectives: The objective of the study was to see the usefulness of LED fluorescent microscopy in the diagnosis of pulmonary tuberculosis.

Methods: This is a prospective study consisted of 150 sputum samples from the patients of NIDCH, Mohakhali. All samples were stained by auramine and ZN stain at BSMMU and culture was done in Lowenstein-Jensen (L-J) media as gold standard at NTRL, Mohakhali.

Results: In this study total 66(44%) out of 150 sputum specimens were positive for Mycobacterium Tuberculosis by culture. Sensitivity and specificity documented for the different modalities were 95.38% and 94.11%, respectively, for the LED assessment; 68.18% and 90.47%, respectively, for the CFM assessment; and 56.06% and 97.61%, respectively, for brightfield microscopy by ZN stain. The difference in their case detection rate was statistically significant (χ2=119.38, p<0.001).

Conclusion: Fluorescence Microscopy (FM) is more sensitive than ZN for diagnosis of pulmonary tuberculosis. However, since FM is more sensitive and rapid, using this method (LED) in clinical laboratories with large specimen numbers is recommended.

DOI: http://dx.doi.org/10.3329/bmrcb.v37i1.7792

Bangladesh Med Res Counc Bull 2011; 37: 7 -10

Downloads

Download data is not yet available.
Abstract
201
PDF
135

Downloads

How to Cite

Khatun, Z., Kamal, M., Roy, C., Sultana, T., Rahman, M., Azad, M., & Ahmed, A. (2011). Usefulness of Light Emitting Diode (LED) fluorescent microscopy as a tool for rapid and effective method for the diagnosis of pulmonary tuberculosis. Bangladesh Medical Research Council Bulletin, 37(1), 7–10. https://doi.org/10.3329/bmrcb.v37i1.7792

Issue

Vol. 37 No. 1 (2011)

Section

Research Papers

License

Authors who publish with this journal agree to the following terms.

Authors retain copyright and grant the journal right of first publication.

Creative Commons Licence
Articles in the Bangladesh Medical Research Council Bulletin are licensed under a Creative Commons Attribution 4.0 International License (CC-BY) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).