Correlation between Circulating Cell Free DNA and Severity of Acute Ischemic Stroke

Cell-free DNA and stroke severity

Authors

  • Nazia Tannim Department of Biochemistry, United Medical College and Hospital, Dhaka.
  • Nowrose Jahan Department of Biochemistry, Medical College for women and Hospital, Dhaka.
  • Jahid Hasan Shovon Armed Forces Institute of Pathology, Dhaka Cantonment, Dhaka
  • Tanjida Ruba Lita Dhaka Medical College Hospital, Dhaka.
  • Ifat Ara Begum Department of Biochemistry, Sir Salimullah Medical College and Hospital, Dhaka.
  • Manindronath Roy Department of Biochemistry, United Medical College and Hospital, Dhaka.

DOI:

https://doi.org/10.3329/bmrcb.v51i02.83828

Keywords:

Circulating cell free DNA (ccfDNA), acute ischemic stroke, National Institutes of Health Stroke Scale (NIHSS) score, stroke severity

Abstract

Background: Acute ischemic stroke management has advanced considerably, but still identifying a reliable biomarker to predict stroke severity and to optimize treatment remains challenging.

Objective: The objective of this study was to assess the correlation between plasma circulating cell free DNA (ccfDNA) and the severity of acute ischemic stroke based on National Institutes of Health Stroke Scale (NIHSS) score.

Methods: This cross-sectional study was carried out from March 2023 to January 2024, at the department of Biochemistry, Sir Salimullah Medical College, Dhaka, Bangladesh. A total 36 patients with ischemic stroke were selected based on clinical and neuroradiological findings within 72 hours of the event. At the time of admission, a clinical assessment was conducted using the NIHSS score. After extracting the ccfDNA from plasma it’s purity and the quantity was initially observed using a Nanodrop spectrophotometer. Finally, to validate the extraction and quantification process, ccfDNA was again measured using TaqMan-based real-time PCR targeting the beta globin gene, where the cycle threshold values were converted to kilogenome equivalents per liter.

Results: Patients with higher NIHSS score had larger amounts of ccfDNA (p-value <0.05). Additionally, a strong positive correlation (r = 0.643; p<0.05) was observed between the NIHSS score and the amount of ccfDNA following ischemic stroke.

Conclusion: Plasma ccfDNA level progressively rises with the severity of damage in acute ischemic stroke.

Bangladesh Medical Res Counc Bull 2025;51(2): 86-90

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Published

2025-11-20

Issue

Vol. 51 No. 2 (2025)

Section

Research Papers

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Copyright (c) 2025 Nazia Tannim, Nowrose Jahan, Jahid Hasan Shovon, Tanjida Ruba Lita, Ifat Ara Begum, Manindronath Roy

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