Development and Validation of a UV-Spectrophotometric Method for Determination of Vildagliptin and Linagliptin in Bulk and Pharmaceutical Dosage Forms

Authors

  • Sujan Banik Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814
  • Palash Karmakar Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814
  • Md Anowar Hossain Miah Department of Product Development, NIPRO JMI Pharma Ltd., Shantibag, Dhaka-1217

DOI:

https://doi.org/10.3329/bpj.v18i2.24316

Keywords:

Type2 diabetes, vildagliptin, linagliptin, UV-spectrophotometer, method validation

Abstract

The present study was undertaken to develop a spectrophotometric method for determination of vildagliptin and Linagliptin in pharmaceutical dosage forms. This paper describes a simple, rapid, accurate and precise UVspectrophotometric method for the assay of vildagliptin and linagliptin in bulk and marketed tablet dosage forms. The validation of the developed method was carried out according to ICH guidelines with respect to linearity, precision, accuracy, specificity, limit of detection and limit of quantification. Calibration curves were obtained in the concentration range of 8-32 ?g/ml for vildagliptin and 5-25 ?g/ml for linagliptin with good correlation coefficients (r=0.999). The precisions of the new method for both drugs were less than the maximum allowable limit (%RSD < 2.0) specified by the USP, ICH and FDA. Therefore, the method was found to be an accurate, reproducible and sensitive for analysis of vildagliptin and linagliptin in pharmaceutical dosage forms.

Bangladesh Pharmaceutical Journal 18(2): 163-168, 2015

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Author Biography

Sujan Banik, Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814



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Published

2015-07-26

How to Cite

Banik, S., Karmakar, P., & Miah, M. A. H. (2015). Development and Validation of a UV-Spectrophotometric Method for Determination of Vildagliptin and Linagliptin in Bulk and Pharmaceutical Dosage Forms. Bangladesh Pharmaceutical Journal, 18(2), 163–168. https://doi.org/10.3329/bpj.v18i2.24316

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