Isolation, Characterization and Antimicrobial Activities of the Metabolites from Different Sources of Bangladesh

Abstract

Antibiotic resistance and emergence of new infections are becoming serious health concern in the recent days. To overcome these problems, the present study was designed to isolate and characterize new microbial species from different locations of Rajshahi division, Bangladesh that can produce bioactive metabolites effective against resistant microorganisms. A total of 56 microbial isolates were obtained by cross streaking and agar disc diffusion method and tested against Bacillus cereus, Staphylococcus aureus ATCC-259233, Listeria monocytogenes, Agrobacterium spp., Escherichia coli FPFC-1407, Shigella dysenteriae AL-35587, Sh. sonnei, and Sh. boydii. Among the total 56 isolates, only 25% were found to inhibit the test pathogens. One of the bacterial isolates designated as DADA-1AIMR-24 revealed maximum antimicrobial activities. From the morphological, cultural and physiological characteristics, pattern of utilization of carbon sources, growth pattern and gram staining, it was concluded that DADA-1AIMR-24 belongs to round shaped gram positive bacteria which is greyish green in color.

Bangladesh Pharmaceutical Journal 20(2): 172-179, 2017