Induction of White Spot Enamel Lesion by S. mutans Biofilm in an Artificial Mouth System and Quantification by QLF

Authors

  • Shamim Sultana Assistant Professor & Head, Dept. of Oral Anatomy & Physiology Pioneer Dental College, Dhaka
  • Md Asafuzzoha Conservative Dentistry & Endodontics BSMMU, Dhaka
  • Hasibul Hasan Assistant Professor Dept. of Conservative Dentistry & Endodontics Pioneer Dental College, Dhaka
  • Md Sajedul Asif Farzan Assistant Professor Dept of Children Preventive & Community Dentistry Pioneer Dental College, Dhaka
  • Khairul Matin Cariology and Operative Dentistry Department of Restorative Sciences COE program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical and Dental University, Tokyo

DOI:

https://doi.org/10.3329/cdcj.v9i1.11829

Keywords:

QLF, White Spot Enamel Lesion (WSEL), biofilm, S. mutans

Abstract

Objective: Aspects of an Artificial Mouth System (AMS) to model the caries process in bovine enamel biofilm induced white spot enamel lesions (WSEL) were analyzed.

Methods: Square shaped and polished bovine enamel slabs were covered with modeling wax and 2.5 mm diameter window was prepared to expose enamel surface at the center of each slab. Artificial biofilms were grown inside an AMS on the slabs using freshly cultured S. mutans (MT8148) in suspended in phosphate buffered saline (PBS), heart infusion (HI) with 1% sucrose and PBS for 20, 30 and 40 hrs at 37°C. The severity of demineralization was quantified by Quantitative Light-induced Fluorescence (QLF, Inspektor, Netherlands). All experiments were repeated three times (n=5 for each group).

Results: A circular WSEL could clearly be detected at the center of each slab by naked eyes, which was prominent after dehydration by mild air drying. QLF data showed that severity of demineralization occurred with the increase of time interval, after 40 hours both ?F (23+/-2.9) and ?Q (148.75+/- 26.78) were significantly more compared to ?F (10.14+/-2.3) and ?Q (47.63+/-30.67) of 20 hours.

Conclusion: An advanced in vitro model of biolfilm induced WSEL was developed that showed promises to be useful in studying enamel demineralization.

DOI: http://dx.doi.org/10.3329/cdcj.v9i1.11829

City Dental College Journal Vol.9(1) 2012 4-6

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Published

2012-09-07

How to Cite

Sultana, S., Asafuzzoha, M., Hasan, H., Farzan, M. S. A., & Matin, K. (2012). Induction of White Spot Enamel Lesion by S. mutans Biofilm in an Artificial Mouth System and Quantification by QLF. City Dental College Journal, 9(1), 4–6. https://doi.org/10.3329/cdcj.v9i1.11829

Issue

Vol. 9 No. 1 (2012)

Section

Original Articles