Optimizing Chlorella Vulgaris And Anabaena Variabilis Growth Conditions For Use As Biofuel Feedstock

Authors

  • NJ Tarin Department of Soil, Water and Environment, University of Dhaka, Dhaka-1000, Bangladesh
  • NM Ali Department of Soil, Water and Environment, University of Dhaka, Dhaka-1000, Bangladesh
  • AS Chamon Department of Soil, Water and Environment, University of Dhaka, Dhaka-1000, Bangladesh
  • MN Mondol Department of Soil, Water and Environment, University of Dhaka, Dhaka-1000, Bangladesh
  • MM Rahman Department of Soil, Water and Environment, University of Dhaka, Dhaka-1000, Bangladesh
  • A Aziz Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

DOI:

https://doi.org/10.3329/jasbs.v42i2.46222

Keywords:

Microalgae, Chlorella vulgaris, Anabaena variabilis, Feedstock, Biofuel, Growth optimization

Abstract

Isolation and characterization of Chlorella vulgaris (green alga) and Anabaena variabilis (cyanobacterium) were made from natural and artificial water bodies of Dhaka University and Khulna, Bangladesh from March through December 2014 using modified Chu-10D medium to determine their potential as feedstock for biofuel production. Optimum growth measured as total chlorophyll and optical density under varying physical and chemical environments was determined. The optimum growth for C. vulgaris was obtained at pH 6.5 under light intensity of 110 μE m-2 s-1 and one and a half times the concentration of the Chu-10D. Compared to this, the optimum growth for A. variabilis was obtained at 7.0 pH, 90 μE m-2 s-1 light intensity and normal Chu 10D. Both organisms were grown at 25o C temperature. Aeration of medium showed a significant positive growth for both the isolates. Supplementation of medium with vitamin B1, B6, B7 and B12 would yield higher biomass of C. vulgaris as biofuel feedstock. Vitamins were not required for growing A. variabilis.

Asiat. Soc. Bangladesh, Sci. 42(2): 191-200, December 2016

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Published

2016-12-25

How to Cite

Tarin, N., Ali, N., Chamon, A., Mondol, M., Rahman, M., & Aziz, A. (2016). Optimizing Chlorella Vulgaris And Anabaena Variabilis Growth Conditions For Use As Biofuel Feedstock. Journal of the Asiatic Society of Bangladesh, Science, 42(2), 191–200. https://doi.org/10.3329/jasbs.v42i2.46222

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