Goat milk as a non-invasive sample for confirmation of Mycobacterium avium subspecies paratuberculosis by IS900 PCR

Authors

  • Bharathy Sukumar Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07
  • Lakshmanasami Gunaseelan Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07
  • Kannan Porteen Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07
  • Karuppanasamy Prabu Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07

Keywords:

Diagnostic test, Excretors, Goat milk, MAP

Abstract

Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne's disease (JD) in cattle, sheep, goats and other ruminants, and Crohns disease in humans. MAPs are shed to external environment through feces and milk. The present study was aimed to evaluate the utility of milk as a non-invasive sample in stage II MAP infections in goats using IS900 polymerase chain reaction (PCR) and sequencing analysis. A total of 32 milk samples from lactating does were collected. Within these 32 milk samples, 15 were collected from pre-confirmed JD positive goats. By IS900 PCR, all the 15 (100%) known JD positive goat milk samples revealed the presence of MAP. However, no unknown goat was identified as MAP positive. The results of this study established the usefulness of milk as a non-invasive sample in screening and confirmation of stage II MAP infection in goats.

DOI:  http://dx.doi.org/10.5455/javar.2014.a25

J. Adv. Vet. Anim. Res., 1(3): 136-139, September 2014

 

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Author Biography

Bharathy Sukumar, Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07

Assistant Professor, Department of Microbiology and Hygiene, FVS, BAU, Mymensingh-2202, Bangladesh

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Published

2014-07-19

How to Cite

Sukumar, B., Gunaseelan, L., Porteen, K., & Prabu, K. (2014). Goat milk as a non-invasive sample for confirmation of Mycobacterium avium subspecies paratuberculosis by IS900 PCR. Journal of Advanced Veterinary and Animal Research, 1(3), 136–139. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/19630

Issue

Vol. 1 No. 3 (2014)

Section

Short Communications

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