Goat milk as a non-invasive sample for confirmation of Mycobacterium avium subspecies paratuberculosis by IS900 PCR

Authors

  • Bharathy Sukumar Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07
  • Lakshmanasami Gunaseelan Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07
  • Kannan Porteen Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07
  • Karuppanasamy Prabu Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07

Keywords:

Diagnostic test, Excretors, Goat milk, MAP

Abstract

Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne's disease (JD) in cattle, sheep, goats and other ruminants, and Crohns disease in humans. MAPs are shed to external environment through feces and milk. The present study was aimed to evaluate the utility of milk as a non-invasive sample in stage II MAP infections in goats using IS900 polymerase chain reaction (PCR) and sequencing analysis. A total of 32 milk samples from lactating does were collected. Within these 32 milk samples, 15 were collected from pre-confirmed JD positive goats. By IS900 PCR, all the 15 (100%) known JD positive goat milk samples revealed the presence of MAP. However, no unknown goat was identified as MAP positive. The results of this study established the usefulness of milk as a non-invasive sample in screening and confirmation of stage II MAP infection in goats.

DOI:  http://dx.doi.org/10.5455/javar.2014.a25

J. Adv. Vet. Anim. Res., 1(3): 136-139, September 2014

 

Downloads

Download data is not yet available.
Abstract
152
PDF
90

Author Biography

Bharathy Sukumar, Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07

Assistant Professor, Department of Microbiology and Hygiene, FVS, BAU, Mymensingh-2202, Bangladesh

Downloads

Published

2014-07-19

How to Cite

Sukumar, B., Gunaseelan, L., Porteen, K., & Prabu, K. (2014). Goat milk as a non-invasive sample for confirmation of Mycobacterium avium subspecies paratuberculosis by IS900 PCR. Journal of Advanced Veterinary and Animal Research, 1(3), 136–139. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/19630

Issue

Vol. 1 No. 3 (2014)

Section

Short Communications

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).