Molecular detection of Mycobacterium tuberculosis from bovine milk samples

Authors

  • V. BhanuRekha Department of Veterinary Public Health and Epidemiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry
  • L. Gunaseelan Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07
  • Ganesh Pawar Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07
  • Reza Nassiri International Institute of Health, Michigan State University, East Lansing, MI 48824
  • Sukumar Bharathy Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Tirupathi

Keywords:

Bovine milk, Mycobacterium tuberculosis, Multiplex PCR, Reverse zoonoses

Abstract

Mycobacterium tuberculosis and Mycobacterium bovis are the major causes of tuberculosis. These may infect many animal species, and are likely to be the main source of infection in humans. A total of 181 bovine raw milk samples and 123 pre-scapular lymph node biopsy samples were collected and subjected to acid fast staining, fluorescent staining, isolation and identification. Genus specific PCR to identify the Mycobacterium tuberculosis complex (MTBC) organism, and multiplex PCR (mPCR) were done to differentiate M. tuberculosis and M. bovis. Among the milk samples tested, only one sample was culturepositive for M. tuberculosis. Four samples were positive by MTBC-PCR and mPCR; all these four were proved to be M. tuberculosis. It is quite likely that animals can be infected with human-originated M. tuberculosis, which in turn may act as a source of infection in humans, becoming a reverse zoonosis. Hence, control strategies for human tuberculosis caused by M. tuberculosis should necessarily include the control strategies in animals too.

http://dx.doi.org/10.5455/javar.2015.b44

 

Downloads

Download data is not yet available.
Abstract
153
PDF
70

Downloads

Published

2014-12-22

How to Cite

BhanuRekha, V., Gunaseelan, L., Pawar, G., Nassiri, R., & Bharathy, S. (2014). Molecular detection of Mycobacterium tuberculosis from bovine milk samples. Journal of Advanced Veterinary and Animal Research, 2(1), 80–83. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/21271

Issue

Vol. 2 No. 1 (2015)

Section

Short Communications

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).