Molecular characterization of Duck Plague virus isolated from Bangladesh

Authors

  • Md. Mostakin Ahamed Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Muhammad Tofazzal Hossain Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Marzia Rahman Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • K. H. M. Nazmul Hussain Nazir Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Mohammad Ferdousur Rahman Khan Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Md. Shafiullah Parvej Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Wahedul Karim Ansari Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Meher Negar Noor-A-Alahi Chiste Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Khaled Bin Amin Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Md. Liakot Hossen Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Sultan Ahmed Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • M. Bahanur Rahman Department of Microbiology and Hygiene, FVS, BAU, Mymensingh

Keywords:

gC gene, Isolation, Identification, PCR, PHA test, DNA polymerase gene

Abstract

Duck plague (DP) is the most feared duck disease in the world. For isolation, identification, molecular detection and characterization of DP virus (DPV), a total of 94 samples were collected from commercial farms (n=6) and households (n=13) from Rajshahi (n=37), Netrokona (n=35) and Mymensingh (n=22) districts of Bangladesh. The samples were processed and inoculated into 11-13 days old embryonated duck eggs for virus propagation. Virus was identified using agar gel immunodiffusion test (AGIT) and passive hemagglutination (PHA) test, and was confirmed by polymerase chain reaction (PCR) targeting DNA polymerase and gC genes, followed by sequencing. Pathogenicity tests were performed using duck embryos, ducklings and ducks. Among the 94 samples, 17 isolates were confirmed as DPV by PCR amplification of partial DNA polymerase (446-bp) and gC genes (78-bp), respectively. One of the isolates (Anatid herpes 1 BAU DMH) was sequenced and found to be closely related with a Chinese variant of DPV (GenBank: JQ647509.1). Thus, we assume that both Bangladeshi and Chinese isolates of DPV may have a common ancestor.

http://dx.doi.org/10.5455/javar.2015.b90

 

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Published

2015-09-07

How to Cite

Ahamed, M. M., Hossain, M. T., Rahman, M., Nazir, K. H. M. N. H., Khan, M. F. R., Parvej, M. S., Ansari, W. K., Chiste, M. N. N.-A.-A., Amin, K. B., Hossen, M. L., Ahmed, S., & Rahman, M. B. (2015). Molecular characterization of Duck Plague virus isolated from Bangladesh. Journal of Advanced Veterinary and Animal Research, 2(3), 296–303. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/24838

Issue

Vol. 2 No. 3 (2015)

Section

Original Articles

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