Pharmacodynamic interaction of fenugreek with insulin and glimepiride in streptozotocin-induced oxidative stress in Sprague Dawley rats

Authors

  • Haritha Chiluka Department of Pharmacology & Toxicology, College of Veterinary Science, Rjendranagar, Hyderabad-30, Telangana
  • Gopala Reddy Alla Department of Animal Nutrition, College of Veterinary Science, Rjendranagar, Hyderabad-30, Telangana
  • Ramana Reddy Yerradoddi Department of Animal Nutrition, College of Veterinary Science, Rjendranagar, Hyderabad-30, Telangana,
  • Anil Kumar Banothu Department of Pharmacology & Toxicology, College of Veterinary Science, Rjendranagar, Hyderabad-30, Telangana

Keywords:

Fenugreek, Glimepiride, Insulin, Oxidative stress, Protein carbonyls, TBARS

Abstract

This study was aimed to assess the effect of
fenugreek-insulin-glimepiride interaction on
oxidative stress in streptozotocin-induced diabetic
rats. A total of 56 male Sprague-Dawley rats were
randomly divided into 7 groups (n=8); group 1: served
as Sham, group 2: Diabetic control, groups 3, 4 and 5:
served as individual treatment group, groups 6 and 7:
treated with combination of insulin-fenugreek and
glimepiride-fenugreek, respectively. Serum
creatinine levels of the rats were estimated at 4th and
8th weeks during treatment. Thiobarbituric Acid
Reactive Substances (TBARS), Glutathione (GSH)
and protein carbonyls were estimated in the kidney
homogenate, and relative kidney weights were
measured at the end of the experiment. Present study
indicated that the levels of TBARS and protein
carbonyls significantly increased in group 2 and
decreased in groups 3 to 7. On the other hand, groups
6 and 7 showed significantly lowered values
compared to the individual treatment groups. The
concentration of GSH was significantly decreased in
group 2 and significantly increased in groups 3 to 7,
and group 7 showed significantly higher
concentration among all the treated groups. The
serum creatinine concentration in group 2 was
significantly higher and all treatment groups (3 to 7)
showed significantly lowered values at 4th and 8th
wks after treatment. The individual treatment groups
(3, 4 & 5), antagonised the significant alteration in the
antioxidant parameters, and their combination was
revealed synergism by improving the oxidative status
in diabetic rats.

 

http://dx.doi.org/10.5455/javar.2015.b89

 

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Published

2015-09-07

How to Cite

Chiluka, H., Alla, G. R., Yerradoddi, R. R., & Banothu, A. K. (2015). Pharmacodynamic interaction of fenugreek with insulin and glimepiride in streptozotocin-induced oxidative stress in Sprague Dawley rats. Journal of Advanced Veterinary and Animal Research, 2(3), 353–356. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/24892

Issue

Vol. 2 No. 3 (2015)

Section

Short Communications

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