Molecular detection of Salmonella spp. isolated from apparently healthy pigeon in Mymensingh, Bangladesh and their antibiotic resistance pattern

Authors

  • Md. Khaled Saifullah Department of Microbiology and Hygiene, FVS, BAU, Mymensingh-2202
  • Md. Montasir Mamun Department of Microbiology and Hygiene, FVS, BAU, Mymensingh-2202
  • Reza Md. Rubayet Department of Microbiology and Hygiene, FVS, BAU, Mymensingh-2202
  • K. H. M. Nazmul Hussain Nazir Department of Microbiology and Hygiene, FVS, BAU, Mymensingh-2202
  • Khalada Zesmin Department of Microbiology and Hygiene, FVS, BAU, Mymensingh-2202
  • Md. Tanvir Rahman Department of Microbiology and Hygiene, FVS, BAU, Mymensingh-2202

Keywords:

Apparently Healthy, Antibiogram, PCR, Pigeons, Salmonella spp.

Abstract

Objectives: Here we determined the prevalence of Salmonella in cloacal swabs and pharyngeal swabs of apparently healthy pigeons sold in the live bird markets and villages in and around Bangladesh Agricultural University Campus, Mymensingh, Bangladesh. 

Materials and methods: A total of 50 samples, comprised of cloacal swabs (n=24) and pharyngeal swabs (n=26) were collected. The samples were processed, and Salmonella was isolated through a series of conventional bacteriological techniques and biochemical tests followed by polymerase chain reaction (PCR). 

Results: The prevalence rate of Salmonella was found to be 37.5% (n=9/24) in cloacal swabs and 30.77% (n=8/26) in pharyngeal swabs with an overall prevalence rate of 34% (n=17/50). The prevalence rate of Salmonella pigeon varied slightly among locations; 34.62% (n=9/26) in live bird markets, and 33.33% (n=8/24) in villages. Molecular detection of 17 Salmonella isolates obtained from biochemical test was performed by genus specific PCR, where all of them amplified a region of 496-bp segment of the histidine transport operon gene. Antibiogram study revealed multi-drug resistant traits in most of the isolates tested. The highest resistance was found against Ampicillin (88.23%) followed by Cephalexin (82.35%). The rate of sensitivity of the isolates to Ciprofloxacin was 100% followed by Azithromycin (82.35%), Gentamicin (76.47%) and Nalidixic acid (76.47%). 

Conclusion: Our findings suggest that pigeons carry multi-drug resistant Salmonella that may transfer to the humans and animals.

http://dx.doi.org/10.5455/javar.2016.c131

Downloads

Download data is not yet available.
Abstract
698
PDF
706

Downloads

Published

2016-04-15

How to Cite

Saifullah, M. K., Mamun, M. M., Rubayet, R. M., Nazir, K. H. M. N. H., Zesmin, K., & Rahman, M. T. (2016). Molecular detection of Salmonella spp. isolated from apparently healthy pigeon in Mymensingh, Bangladesh and their antibiotic resistance pattern. Journal of Advanced Veterinary and Animal Research, 3(1), 51–55. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/27208

Issue

Vol. 3 No. 1 (2016)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).