Neutrophil surface adhesion molecule and toll like receptor dynamics in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis

Authors

  • Dilip Kumar Swain Lactation and Immuno Physiology Laboratory, National Dairy Research Institute, Karnal 132 001, Haryana
  • Mohar Singh Kushwah Lactation and Immuno Physiology Laboratory, National Dairy Research Institute, Karnal 132 001, Haryana
  • Ajay Kumar Dang Lactation and Immuno Physiology Laboratory, National Dairy Research Institute, Karnal 132 001, Haryana

Keywords:

CD11b, CD62L, Crossbred cows, Mastitis, Neutrophils, TLR

Abstract

Objective: The present study was an attempt to delineate the roles played by the neutrophil surface adhesion molecules and toll like receptors (TLRs) in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis.

Materials and methods:  Thirty six Karan Fries (KF) cows were categorized into three groups namely healthy (n=12), subclinical mastitis (SCM; n=12) and clinical mastitis (CM; n=12) after screening 146 cows. The grouping was done based on evaluation of collected milk samples by routine procedures like Californian Mastitis Test (CMT) scoring, microscopic counting of milk cells (SCC), bacterial culture of milk samples and observing gross changes in milk. Culture of milk and blood was done for the identification of Staphylococcus aureus subclinical and clinical mastitis.

Results: Healthy cows expressed significantly (P<0.05) higher L-selectin (CD62L) in both milk and blood neutrophils as compared to the animals suffering from SCM and CM; however, no significant difference was noticed between milk and blood neutrophils. Significant (P<0.05) increase in the expression of beta integrin (CD11b) was observed in the CM group of cows as compared to SCM and healthy cows. Similar trend in the expressions of TLR2 and TLR4 in both blood and milk neutrophils was observed in the CM cows as compared to the healthy and SCM cows. Milk neutrophils revealed a higher expression of TLR as compared to blood neutrophils.

Conclusion: Host elicits stage specific expression of surface adhesion molecules and TLR2 and TLR4 as dynamic host innate immune response against Staphylococcal mastitis.

http://doi.org/10.5455/javar.2016.c136

Downloads

Download data is not yet available.
Abstract
51
PDF
24

Downloads

Published

2016-07-25

How to Cite

Swain, D. K., Kushwah, M. S., & Dang, A. K. (2016). Neutrophil surface adhesion molecule and toll like receptor dynamics in crossbred cows suffering from Staphylococcus aureus subclinical and clinical mastitis. Journal of Advanced Veterinary and Animal Research, 3(2), 99–105. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/28859

Issue

Vol. 3 No. 2 (2016)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).