Isolation, identification and antimicrobial resistance profile of Staphylococcus aureus in Cockroaches (Periplaneta americana)

Authors

  • Ariful Islam EcoHealth Alliance, New York, NY, USA. & Institute of Epidemiology, Disease Control and Research (IEDCR), Mohakhali-1212
  • Aurjun Deb Nath Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 4225
  • Kamrul Islam Institute of Epidemiology, Disease Control and Research (IEDCR), Mohakhali-1212
  • Shariful Islam EcoHealth Alliance, New York, NY, USA. & Institute of Epidemiology, Disease Control and Research (IEDCR), Mohakhali-1212
  • Shovon Chakma Institute of Epidemiology, Disease Control and Research (IEDCR), Mohakhali-1212
  • Muhammad Belal Hossain International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Mohakhali-1212
  • Abdullah Al-Faruq Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 4225
  • Mohammad Mahmudul Hassan Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 4225

Keywords:

Antibiogram, Cockroach, External washing, Gut content, Prevalence, Resistance

Abstract

Objective: The study was conducted to determine the prevalence of Staphylococcus aureus in cockroaches (Periplaneta americana), and to assess the antimicrobial resistance profiles of the isolated bacteria.

Materials and methods: A total of 150 cockroaches (P. americana) were randomly captured from three households and four restaurants in Chittagong City Corporation, Bangladesh during July to December 2014. The cockroaches were transported to the bacteriology laboratory at the Poultry Research and Training Centre (PRTC), Chittagong Veterinary and Animal Sciences University. The isolation and identification of Staphylococcus spp. from the external surface wash and gut homogenates by pooling cockroaches were done by following conventional bacteriological examinations followed by biochemical characterization. The antibiotic susceptibility profiles of the isolates were determined using disc diffusion method. 

Results: In this study, the overall prevalence of S. aureus was 38% (n=57/150). Higher prevalence of Staphylococcus spp. was observed among the cockroaches from restaurant (49.3%; n=37/75) as compared to those of households (26.7%; n=20/75) having a significant difference (P<0.05). Highest level of resistance by the Staphylococcus spp. was found to Penicillin (68%) followed by Erythromycin (60%), Oxacillin (46%) and Clindamycin (31%). On the other hand, the Staphylococci isolates were highly sensitive to Cephalothin (84%) and Kanamycin (65%).

Conclusion: The rational use of antibiotics needs to be adopted in both human and animal medicine practices to prevent the emergence of drug resistant Staphylococcus spp.

http://doi.org/10.5455/javar.2016.c153

Downloads

Download data is not yet available.
Abstract
28
PDF
35

Author Biographies

Ariful Islam, EcoHealth Alliance, New York, NY, USA. & Institute of Epidemiology, Disease Control and Research (IEDCR), Mohakhali-1212


Aurjun Deb Nath, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 4225


Kamrul Islam, Institute of Epidemiology, Disease Control and Research (IEDCR), Mohakhali-1212


Shovon Chakma, Institute of Epidemiology, Disease Control and Research (IEDCR), Mohakhali-1212


Muhammad Belal Hossain, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Mohakhali-1212


Abdullah Al-Faruq, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 4225


Mohammad Mahmudul Hassan, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 4225


Downloads

Published

2016-11-03

How to Cite

Islam, A., Nath, A. D., Islam, K., Islam, S., Chakma, S., Hossain, M. B., Al-Faruq, A., & Hassan, M. M. (2016). Isolation, identification and antimicrobial resistance profile of Staphylococcus aureus in Cockroaches (Periplaneta americana). Journal of Advanced Veterinary and Animal Research, 3(3), 221–228. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/30140

Issue

Vol. 3 No. 3 (2016)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).