Screening of antibiotic residues in chicken meat in Bangladesh by thin layer chromatography

Authors

  • Yousuf Ali Sarker
  • Md. Mehedi Hasan
  • Torun Kumar Paul
  • Sm. Zamiul Rashid
  • Md. Nurul Alam
  • Mahmudul Hasan Sikder

Keywords:

Antibiotic residues, Broiler, Liver, Meat, TLC

Abstract

Objective: Screening of antibiotic residues in broiler chicken meat and liver collected from farms and local markets in Bangladesh.

Materials and methods: A total of 160 samples (breast, thigh muscle and liver) were collected from markets and farms from different region of Bangladesh. PBS buffer system with trichloracetic acid and diethyl ether based sample extraction was performed. For comparison the standard antibiotics; Ciprofloxacin (CIP), Enrofloxacin (ENR), Oxytetracycline (OTC), Amoxicillin (AMOX) and Doxycycline (DOX) were prepared by dissolving in methanol. Samples were pointed on TLC plates transferred to TLC tank containing acetone-methanol (1:1) as mobile phase. Retention factor (Rf) was calculated after observing the chromatograms on UV light at 256 nm. Same Rf value of standard and sample considered similar compound.

Results: Liver sample were mostly positive for antibiotics residue followed by thigh muscles and breast muscle. The frequency of antibiotic residues was highest in liver followed by thigh muscles and breast muscle. Among the antibiotics CIP ranked top in all types of sample. In breast muscle highest antibiotic was CIP (39%) followed by DOX (26%), AMOX (24%), OTC (23%) and lowest was ENR (21%). In thigh muscle, 42, 29, 28, 27 and 24% sample was positive for CIP, OTC, DOX, AMOX and ENR, respectively. Highest number of liver samples were shown positive result for all screened antibiotics (CIP-52%, OTC-46%, DOX-43%, AMOX-42% and ENR-36%).

Conclusion: This study ascertained those antibiotics residues are present in chicken muscle and liver which causes serious health hazards to consumers. So proper steps should be taken to control emergence of antibiotic resistance in human being as well as in the environment.

http://doi.org/10.5455/javar.2018.e257     

Downloads

Download data is not yet available.
Abstract
53
PDF
46

Author Biographies

Yousuf Ali Sarker

Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Md. Mehedi Hasan

Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Torun Kumar Paul

Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Sm. Zamiul Rashid

Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Md. Nurul Alam

Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Mahmudul Hasan Sikder

Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Downloads

Published

2018-06-28

How to Cite

Sarker, Y. A., Hasan, M. M., Paul, T. K., Rashid, S. Z., Alam, M. N., & Sikder, M. H. (2018). Screening of antibiotic residues in chicken meat in Bangladesh by thin layer chromatography. Journal of Advanced Veterinary and Animal Research, 5(2), 140–145. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/37159

Issue

Vol. 5 No. 2 (2018)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).