Antibody against synthetic peptide of prolactin-inducible protein homologue precursor (PIP-HP) of Bali Cattle (Bos javanicus) saliva as a potential biomarker for immunoassay development

Authors

  • Djoko Kisworo
  • Sulaiman Ngongu Depamede

Keywords:

Amino acid, Bactericidal, E. coli, Innate immunity, Lateral flow assay, S. aureus

Abstract

Objective: The present study aimed to produce and analyze antibody against a synthetic amino acid sequence fragment of PIP-HP of Bali cattle saliva.

Materials and Methods: The synthetic amino acid sequence of the PIP-HP (VIRELGICPDDWAVIPIKANRF) was developed, conjugated to bovine serum albumin and was used to immunize Indonesian local rabbits. Positive sera that specific against the PIP-HP were pooled and purified sequentially by implying ammonium sulfate precipitation and protein A affinity methods. Purified antibody was then employed to analyze of PIP-HP in the ruminants’ saliva by means of westernblot assays.

Results: A polyclonal antibody specific to asynthetic amino acid sequence fragment of PIP-HP of Bali cattle saliva was successfully produced. Our results show that the antibody potentially to be used to develop an immuno-diagnostic kit. Furthermore, the antibody was also able to inhibit the growth of both Escherichia coli and Staphylococcus aureus cultures significantly (P<0.001) at concentration of 0.3 mg/mL.

Conclusions: In this study, antibody against synthetic peptide of PIP-HP (VIRELGICPDDWAVIPIKANRF) of Bali cattle saliva origin has been produced successfully. Further studies need to be carried out since the antibody has potential as biomarker for non-invasive immunoassays development.

http://doi.org/10.5455/javar.2018.e264     

Downloads

Download data is not yet available.
Abstract
17
PDF
27

Author Biographies

Djoko Kisworo

1Faculty of Animal Science, University of Mataram.Jl. Majapahit No. 62 Mataram NTB 83125, Indonesia.

Sulaiman Ngongu Depamede

Faculty of Animal Science, University of Mataram.Jl. Majapahit No. 62 Mataram NTB 83125, Indonesia.

Downloads

Published

2018-06-28

How to Cite

Kisworo, D., & Depamede, S. N. (2018). Antibody against synthetic peptide of prolactin-inducible protein homologue precursor (PIP-HP) of Bali Cattle (Bos javanicus) saliva as a potential biomarker for immunoassay development. Journal of Advanced Veterinary and Animal Research, 5(2), 182–187. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/37164

Issue

Vol. 5 No. 2 (2018)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).