The potential use of infrared spectroscopy and multivariate analysis for differentiation of beef meatball from dog meat for Halal authentication analysis

Authors

  • W.S. Rahayu
  • S. Martono
  • Abdul Rohman

Keywords:

Bligh-Dryer, Chemometrics, Dog meat, Folch, FTIR spectroscopy

Abstract

Objective:  The objective of this research was to assess the suitability of FTIR spectroscopy coupled with multivariate analysis of partial least square regression (PLSR) along with pattern recognition technique of principal component analysis (PCA) for rapid quantitative and qualitative (identification) analysis of dog meat in beef meatball formulation.

Materials and Methods: The lipid fraction of meatball was obtained by employing two different extraction techniques, namely Bligh-Dyer and Folch method. FTIR spectral bands correlated with beef fat, pork fat, chicken fat and rat fat were measured, interpreted, and qualitatively analyzed. The small variations among spectra were exploited as a basis tools to differentiate between dog fat and other animal fats. 

Results: PCA at combined wavenumber regions of 1700-700 cm-1 was capable of identifying dog meat in meatball. These wavenumbers were also used for quantitative analysis of dog meat in meatball using PLSR model. Based on statistical parameters used, namely R2, RMSEC and RMSEP, Folch extraction method offered higher R2 and lower RMSEC and RMSEP than Bligh-Dyer. PCA is succesfully applied for classification between meatball containing dog meat and other meats.

Conclusion: FTIR spectroscopy coupled with multivariate analyses of PLSR and PCA was effective means for rapid screening of dog meat in meatball products.

http://doi.org/10.5455/javar.2018.e281

Downloads

Download data is not yet available.
Abstract
624
PDF
432

Author Biographies

W.S. Rahayu

Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia.

 

Faculty of Pharmacy, Muhammadiyah University of Purwokerto. Purwokerto, Indonesia.

S. Martono

Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia.

Abdul Rohman

Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia.

 

Research Center of Halal Products, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia.

Downloads

Published

2018-10-02

How to Cite

Rahayu, W., Martono, S., & Rohman, A. (2018). The potential use of infrared spectroscopy and multivariate analysis for differentiation of beef meatball from dog meat for Halal authentication analysis. Journal of Advanced Veterinary and Animal Research, 5(3), 307–314. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/38436

Issue

Vol. 5 No. 3 (2018)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).