Phytochemical screening, proximate analysis, median lethal dose (LD50), hematological and biochemical effects of various extracts of Abrus precatorius seeds in Mus musculus

Authors

  • Matthew Terzungwe Tion
  • Hanna Fotina
  • Saganuwan Alhaji Saganuwan

Keywords:

Abrus precatorius seed, Blood tonic, Hypocholesterolemic, Immunosuppressant, Renotoxicity

Abstract

Objective: Abrus precatorius is a universal panacea in herbal medicine. In view of this, phytochemical screening, proximate analysis, median lethal dose (LD50), hematological and biochemical effects of extracts of A. precatorius seed was studied in Mus musculus.

Materials and methods: Nineteen (19) mice were used for the study. Four (4) mice were used for determination of median lethal dose of the aqueous and ethyl acetate extracts respectively. The LD50 of aquous and ethyl acetate extracts was estimated at 187.5±62.5 mg/Kg and 175±75 mg/Kg respectively. The remaining fifteen (15) mice divided into 3 groups of 5 each were used for hematological and biochemical studies. Group 1 was administered 1 mL of distilled water while groups 2 and 3 were administered 1/10th (18.75 mg/Kg) of LD50 (187.5 mg/Kg) of methanolic and ethanolic seed extracts, for a period of 4 weeks.

Results: Proximate analysis showed the presence of moisture, ash, crude protein and crude fiber. Carbohydrate and organic matter were calculated. Phytochemical screening showed alkaloids, flavanoids, tannins, saponins, and reducing sugars in both ethanolic and aqueous extracts. Cardiac glycosides were present in aqueous extract. Hematology revealed increased packed cell volume (PCV), hemoglobin (Hb), mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH) whereas red blood cell (RBC) and white blood cell (WBC) were significantly (P<0.05) decreased. Biochemistry revealed significantly decreased (P<0.05) total protein, albumin, cholesterol, globulin and albumin/globulin ratio whereas creatinine and alkaline phosphatase were significantly increased.

Conclusion: A. precatorius seed extracts are very toxic and can be used as blood tonic, immunosupressant, hypocholesterolemic and renotoxic.

http://doi.org/10.5455/javar.2018.e286           

Downloads

Download data is not yet available.
Abstract
28
PDF
42

Author Biographies

Matthew Terzungwe Tion

Department of Veterinary Medicine, College of Veterinary Medicine, University of Agriculture, Makurdi, Benue State, Nigeria.

Hanna Fotina

Sumy National Agrarian University, 160 Kirova Street, Sumy 40021, Ukraine.

Saganuwan Alhaji Saganuwan

Department of Veterinary Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Agriculture, Makurdi, Benue State, Nigeria.

Downloads

Published

2018-10-02

How to Cite

Tion, M. T., Fotina, H., & Saganuwan, S. A. (2018). Phytochemical screening, proximate analysis, median lethal dose (LD50), hematological and biochemical effects of various extracts of Abrus precatorius seeds in Mus musculus. Journal of Advanced Veterinary and Animal Research, 5(3), 354–360. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/38441

Issue

Vol. 5 No. 3 (2018)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).