In vitro studies on gastrointestinal monogastric and avian models to evaluate the binding efficacy of mycotoxin adsorbents by liquid chromatography-tandem mass spectrometry

Authors

  • Jutamas Prapapanpong Department of Veterinary Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand
  • Pareeya Udomkusonsri Department of Veterinary Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand
  • Wiratchanee Mahavorasirikul Drug Discovery and Development Center, Office of Advanced Science and Technology,Thammasat University, Pathumthani 12121, Thailand
  • Sasiprapa Choochuay Department of Veterinary Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand
  • Natthasit Tansakul Department of Veterinary Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand

Keywords:

Aflatoxin B1; deoxynivalenol; LC-MS/ MS; mycotoxin adsorbent; mycotoxin binder; zearalenone

Abstract

Objective: The objective of this study is evaluating the efficacies of 11 mycotoxin adsorbent prod­ucts, marketed in South East Asia. Three prominently occurring mycotoxins; aflatoxin B1 (AFB1), deoxynivalenol (DON), and zearalenone (ZEN) were simultaneously spiked into the samples.

Materials and Methods: Samples were simultaneously tested in vitro in phosphate buffer and simulated at different pH conditions in the gastrointestinal tracts of the porcine and avian model, analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Results: All mycotoxin adsorbent products had high efficacy at over 90% for AFB1 adsorption in both GI porcine and avian models. AFB1 could be adsorbed more in acidic condition than the basic condition. ZEN adsorption was determined to be more stable at pH 3 than pH 6.5 or 8.4, in which pH condition might influence on ZEN desorption rate. DON was poorly adsorbed by all tested agents.

Conclusions: The finding showed that the adsorption rate varied depending on the type of adsorbent. Our results might provide useful information regarding the efficacy of mycotoxin adsorbents commercially marketed in the region.

J. Adv. Vet. Anim. Res., 6(1): 125-132, March 2019

Downloads

Download data is not yet available.
Abstract
21
PDF
18

Downloads

Published

2019-03-31

How to Cite

Prapapanpong, J., Udomkusonsri, P., Mahavorasirikul, W., Choochuay, S., & Tansakul, N. (2019). In vitro studies on gastrointestinal monogastric and avian models to evaluate the binding efficacy of mycotoxin adsorbents by liquid chromatography-tandem mass spectrometry. Journal of Advanced Veterinary and Animal Research, 6(1), 125–132. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/40848

Issue

Vol. 6 No. 1 (2019)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).