Evaluation of wound healing potential of pomegranate (Punica granatum) whole fruit extract on skin burn wound in rats (Rattus norvegicus)

Authors

  • Bambang Sektiari Lukiswanto Department of Clinical Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
  • Alya Miranti Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
  • Sri Agus Sudjarwo Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
  • Hardany Primarizky Department of Clinical Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
  • Wiwik Misaco Yuniarti Department of Clinical Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia

Keywords:

Burn wound; ellagic acid; pomegranate; silver sulfadiazine; wound healing

Abstract

Objective: This research was conducted to study the wound healing process of whole fruit pomegranate extract (punica granatum) standardized with 40% ellagic acid ointment for deep second- degree burn wound of skin in the rat (Rattus norvegicus).

Materials and Methods: Powder of standardized pomegranate extract (SPE) with 40% ellagic acid was processed to become ointments. Twenty-five male rats, weighed 150–180 gm at 3 months of age, were randomly divided into five groups. After anesthetized, stainless circle plate with 1 cm of diameter in 85°C was contacted firmly toward right gluteal of rat skins for 5 sec in order to create deep second-degree burn wound. Control groups consist of (T0) cream base and (T1) 1% silver sulfadiazine. Treatment groups consist of (T2) 2.5% SPE, (T3) 5% SPE, and (T4) 10% SPE. Histopathological preparation used hematoxylin-eosin stained skin samples. Histological observations were performed using the optics microscope against collagen, the number of polymorphonuclear cell (PMN) infiltration, the degree of angiogenesis, and re-epithelization. The results were statistically compared between groups.

Results: Microscopic observation on the wound healing process on the collagen, PMN infiltration, angiogenesis, and re-epithelization showed that topical administration of 10% SPE in burns gives the best result. This is characterized by a high density of collagen with a good arrangement, which is accompanied by a complete and mature epithelium, low number of inflammatory cells, and angiogenesis. This may be caused by the compounds in the pomegranate extract, which have the antioxidant, anti-inflammatory, and anti-bacterial effects.

Conclusion: This study reveals that 10% SPE accelerates the healing of deep second-degree burn wound. Thus, pomegranate standardized with 40% ellagic acid is a promising herb for the healing of burn wound of skin.

J. Adv. Vet. Anim. Res., 6(2): 202-207, June 2019

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Published

2019-06-13

How to Cite

Lukiswanto, B. S., Miranti, A., Sudjarwo, S. A., Primarizky, H., & Yuniarti, W. M. (2019). Evaluation of wound healing potential of pomegranate (Punica granatum) whole fruit extract on skin burn wound in rats (Rattus norvegicus). Journal of Advanced Veterinary and Animal Research, 6(2), 202–207. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/41760

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Section

Short Communications