Genetic diversity and population structure of Echinococcus multilocularis: An in-silico global analysis

Authors

  • Ayed Alshammari Department of Biology, College of Science, University of Hafr Al Batin, Hafr Al Batin, Saudi Arabia
  • Muhammad Irshad Subhani Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan
  • Majed H Wakid Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
  • Abdulsalam A M Alkhaldi Biology Department, College of Science, Jouf University, Sakaka, Saudi Arabia
  • Shujaat Hussain Faculty of Veterinary and Animal Sciences, PMAS Arid Agriculture University, Rawalpindi, Pakistan
  • Muhammad Abdullah Malik Department of Parasitology, University of Agriculture, Faisalabad, Pakistan
  • Muhammad Saqib Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan
  • Warda Qamar Department of Parasitology, University of Agriculture, Faisalabad, Pakistan
  • Mughees Aizaz Alvi Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan

Keywords:

Echinococcus multilocularis, cox1, nad1, genetic variability

Abstract

Objectives: Alveolar echinococcosis is caused by Echinococcus multilocularis, a parasite of zoo­notic significance with a wide range of intermediate and final hosts, and the parasite survives suc­cessfully in diversified conditions. Plentiful studies have been done to study the genetic structure of the population of the parasite and the level of intimate kinship using mitochondrial (mt) DNA. The present study was conducted to investigate the population structure, genetic variation, and phylogenetic relationship of various isolates of E. multiocularis submitted to GenBank worldwide. Sequences of mt genes (mt-cytochrome c oxidase (cox1), mt-NADH dehydrogenase (nad1)) of E. multilocularis were analyzed to achieve the set goals.

Materials and Methods: A total of 275 and 124 gene sequences of mt-cox1 and mt-nad1 belong­ing to E. multilocularis, respectively, were retrieved from the National Center for Biotechnology Information GenBank. The retrieved sequences were subjected to alignment with respective reference sequences using MEGA software. The PopArt software was used to establish medi­an-joining networks, while DnaSp was used to calculate neutrality and diversity indices. MrBayes software was used to investigate the phylogenetic association between haplotypes based on Bayesian phylogeny.

Results: Approximately 13 and 20 distinctive haplotypes of nad1 and cox1 genes, respectively, were observed in the present study. In both of the mt genes, diversity indices indicated low haplo­type (mt-cox1 = 0.140; mt-nad1 = 0.374) and nucleotide (mt-cox1 = 0.00111; mt-nad1 = 0.00287) diversities. The values of Tajima’s D and Fu Fs for a population of both of the genes under study were found to be negative.

Conclusion: This study is a maiden attempt to provide insights into the population structure and genetic variation of E. multilocularis on a global scale. However, it is suggested that to better understand the population structure and genetic diversity of E. multilocularis, more geographical locations and amplifications of full-length gene sequences should be considered, which could be helpful in widening the insights into the genetic diversity of E. multilocularis.

Adv. Vet. Anim. Res., 11(2): 264-274, June 2024

http://doi.org/10.5455/javar.2024.k772

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Published

2024-06-04

How to Cite

Alshammari, A., Subhani, M. I., Wakid, M. H., Alkhaldi, A. A. M., Hussain, S., Malik, M. A., Saqib, M., Qamar, W., & Alvi, M. A. (2024). Genetic diversity and population structure of Echinococcus multilocularis: An in-silico global analysis. Journal of Advanced Veterinary and Animal Research, 11(2), 264–274. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75585

Issue

Vol. 11 No. 2 (2024)

Section

Original Articles

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Copyright (c) 2024 Ayed Alshammari, Muhammad Irshad Subhani, Majed H Wakid, Abdulsalam A M Alkhaldi, Shujaat Hussain, Muhammad Abdullah Malik, Muhammad Saqib, Warda Qamar, Mughees Aizaz Alvi

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