Antibiotic resistance and genotyping of mecA-positive methicillin-resistant Staphylococcus aureus (MRSA) from milk and nasal carriage of dairy water buffaloes (Bubalus bubalis) in the Philippines

Authors

  • Alona T Badua Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
  • Sukolrat Boonyayatra Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
  • Nattakarn Awaiwanont Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
  • Paula Blanca V Gaban Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Nueva Ecija, Philippines.
  • Claro N Mingala Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Nueva Ecija, Philippines.

Keywords:

MRSA; mecA; water buffaloes; SCCmec; spa type; ST type

Abstract

Objective: Mastitis is considered as an economically important disease of dairy buffaloes in Asia. This study examined the mastitis milk and nasal swab samples for the detection and genotyping of methicillin-resistant Staphylococcus aureus (MRSA) in water buffaloes.  Materials and Methods: Staphylococcus aureus was identified based on biochemical tests and Polymerase Chain Reaction (PCR) detection of nuc gene, whereas MRSA on mecA gene. The disc diffusion test was used to determine the antibiotic resistance and staphylococcal cassette chro­mosome mec (SCCmec), spa, and multilocus sequence typing for the genotyping of isolates.  Results: Staphylococcus aureus was detected on 39/93 milk (41.94%) and 27/384 nasal swab (7.03%) samples. However, only nine isolates (23.08%) harbored the mecA gene from milk sam­ples and three isolates (11.11%) from the nasal carriage. All MRSA isolates exhibited resistance to cefoxitin and penicillin, whereas 50% were found resistant to clindamycin. All these isolates were found susceptible to sulfa-trimethoprim and chloramphenicol, whereas the majority of the isolates were susceptible to gentamicin, ciprofloxacin, tetracycline, and rifampicin. The SCCmec types of the MRSA isolates were type IVc (50.00%), type II (8.33%), type I (8.33%), and non-typea­ble (33.33%). The spa types and sequence type (ST) identified were t019 (ST30), t701 (ST1649), t311 (ST5), t657 (ST1148), t015 (ST508), t1939 (ST12), t800 (ST9), t091 (ST2454), t138 (ST5991), and t1642 (ST5992).  Conclusion: Milk and nasal swab samples from dairy water buffaloes were found positive for MRSA. The MRSA isolates were still susceptible to most antibiotics tested. Moreover, the gen­otypes of some MRSA isolates were found similar to some human MRSA strains, suggesting a possible human to animal transmission.

J. Adv. Vet. Anim. Res., 7(3): 397-406, Sep 2020

http://doi.org/10.5455/javar.2020.g434

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Published

2020-06-29

How to Cite

Badua, A. T., Boonyayatra, S., Awaiwanont, N., Gaban, P. B. V., & Mingala, C. N. (2020). Antibiotic resistance and genotyping of mecA-positive methicillin-resistant Staphylococcus aureus (MRSA) from milk and nasal carriage of dairy water buffaloes (Bubalus bubalis) in the Philippines. Journal of Advanced Veterinary and Animal Research, 7(3), 397–406. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75588

Issue

Vol. 7 No. 3 (2020)

Section

Original Articles

License

Copyright (c) 2020 Alona T Badua, Sukolrat Boonyayatra, Nattakarn Awaiwanont, Paula Blanca V Gaban, Claro N Mingala

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