Antibiotic resistance and genotyping of mecA-positive methicillin-resistant Staphylococcus aureus (MRSA) from milk and nasal carriage of dairy water buffaloes (Bubalus bubalis) in the Philippines

Authors

  • Alona T Badua Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
  • Sukolrat Boonyayatra Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
  • Nattakarn Awaiwanont Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
  • Paula Blanca V Gaban Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Nueva Ecija, Philippines.
  • Claro N Mingala Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Nueva Ecija, Philippines.

Keywords:

MRSA; mecA; water buffaloes; SCCmec; spa type; ST type

Abstract

Objective: Mastitis is considered as an economically important disease of dairy buffaloes in Asia. This study examined the mastitis milk and nasal swab samples for the detection and genotyping of methicillin-resistant Staphylococcus aureus (MRSA) in water buffaloes.  Materials and Methods: Staphylococcus aureus was identified based on biochemical tests and Polymerase Chain Reaction (PCR) detection of nuc gene, whereas MRSA on mecA gene. The disc diffusion test was used to determine the antibiotic resistance and staphylococcal cassette chro­mosome mec (SCCmec), spa, and multilocus sequence typing for the genotyping of isolates.  Results: Staphylococcus aureus was detected on 39/93 milk (41.94%) and 27/384 nasal swab (7.03%) samples. However, only nine isolates (23.08%) harbored the mecA gene from milk sam­ples and three isolates (11.11%) from the nasal carriage. All MRSA isolates exhibited resistance to cefoxitin and penicillin, whereas 50% were found resistant to clindamycin. All these isolates were found susceptible to sulfa-trimethoprim and chloramphenicol, whereas the majority of the isolates were susceptible to gentamicin, ciprofloxacin, tetracycline, and rifampicin. The SCCmec types of the MRSA isolates were type IVc (50.00%), type II (8.33%), type I (8.33%), and non-typea­ble (33.33%). The spa types and sequence type (ST) identified were t019 (ST30), t701 (ST1649), t311 (ST5), t657 (ST1148), t015 (ST508), t1939 (ST12), t800 (ST9), t091 (ST2454), t138 (ST5991), and t1642 (ST5992).  Conclusion: Milk and nasal swab samples from dairy water buffaloes were found positive for MRSA. The MRSA isolates were still susceptible to most antibiotics tested. Moreover, the gen­otypes of some MRSA isolates were found similar to some human MRSA strains, suggesting a possible human to animal transmission.

J. Adv. Vet. Anim. Res., 7(3): 397-406, Sep 2020

http://doi.org/10.5455/javar.2020.g434

Downloads

Download data is not yet available.
Abstract
25
PDF
22

Downloads

Published

2020-06-29

How to Cite

Badua, A. T., Boonyayatra, S., Awaiwanont, N., Gaban, P. B. V., & Mingala, C. N. (2020). Antibiotic resistance and genotyping of mecA-positive methicillin-resistant Staphylococcus aureus (MRSA) from milk and nasal carriage of dairy water buffaloes (Bubalus bubalis) in the Philippines. Journal of Advanced Veterinary and Animal Research, 7(3), 397–406. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75588

Issue

Vol. 7 No. 3 (2020)

Section

Original Articles

License

Copyright (c) 2020 Alona T Badua, Sukolrat Boonyayatra, Nattakarn Awaiwanont, Paula Blanca V Gaban, Claro N Mingala

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).