Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles

Authors

  • Ismail Abd Elhafez Radwan Raheel Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
  • Walid Hamdy Hassan Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
  • Shaaban Salem Radwan Salem Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
  • Hala Sayed Hassan Salam Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.

Keywords:

Biofilm E. coli; endometritis; resistance; virulence

Abstract

Objective: The objectives of this study were to determine the biofilm-forming capability and anti­microbial susceptibility of Escherichia coli recovered from bovine endometritis samples.  Materials and Methods: A total of 120 uterine specimens were collected from cows suffering from endometritis for bacteriological examination. Antimicrobial susceptibility testing was carried out for all isolated E. coli by using the disc diffusion method. The isolates were phenotypically studied for biofilm-forming ability by cultivation on yeast extract -casamino acids Congo red agar (CRA). Some randomly selected isolates were chosen for the molecular identification of some virulence and resistance genes.  Results: A total of 58(48.3%) E. coli isolates could be isolated from the 120 samples. Antimicrobial susceptibility testing exhibited that 91.4%, 79.3%, 79.3%, 74.1%, and 58.6% of the isolates were sensitive to gentamicin, amoxicillin-clavulanic acid, ciprofloxacin, cephalexin, and sulfamethox­azole-trimethoprim, respectively. On the other hand, 91.4% and 70.7% isolates were resistant to cefotaxime and doxycycline, respectively. Cultivation on CRA revealed that 46.6% of isolates were biofilm producers. The molecular detection of resistance and virulence genes declared that all iso­lates harbored blaTEM, sul1, tetA, qnrS, blaCTX-M, and fimH with a percentage of 100%, papC (40%), and hlyA (10%). FimH was the most prevalent biofilm-associated gene.  Conclusion: The present study highlights the high prevalence of multi-drug- resistant E. coli asso­ciated with bovine endometritis. The detection of the fimH gene is circumstantial evidenced that this gene has a crucial role in biofilm formation in intrauterine pathogenic E. coli.

J. Adv. Vet. Anim. Res., 7(3): 442-451, Sep 2020

http://doi.org/10.5455/javar.2020.g440

Downloads

Download data is not yet available.
Abstract
50
PDF
25

Downloads

Published

2020-08-05

How to Cite

Radwan Raheel, I. A. E., Hassan, W. H., Salem, S. S. R., & Salam, H. S. H. (2020). Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles. Journal of Advanced Veterinary and Animal Research, 7(3), 442–451. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75608

Issue

Vol. 7 No. 3 (2020)

Section

Original Articles

License

Copyright (c) 2020 Ismail Abd Elhafez Radwan Raheel, Walid Hamdy Hassan, Shaaban Salem Radwan Salem, Hala Sayed Hassan Salam

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).