The effect of Andaliman (Zanthoxylum acanthopodium DC.) fruit extracted with ethanol on TNF-α and TRPA-1 levels in type II diabetes-induced mice

Authors

  • Boyke Marthin Simbolon Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia
  • OK Yulizal Faculty of Medicine, Universitas Prima Indonesia, Medan, Indonesia
  • Albert Manggading Hutapea Department of Physiology, Universitas Advent Indonesi‑a, Indonesia
  • Erwin Handoko Quality Assurance and Educational Development, Murni Teguh University, Deli Serdang, Indonesia

Keywords:

TNF-α; TRPA-1; type 2 diabetes mellitus; Andaliman fruit extract; Zanthoxylum acanthopodium fruit extract

Abstract

Objective: The present study investigated the effects of Andaliman fruit extract on tumor necrosis factor-alpha (TNF-α) and transient receptor potential ankyrin-1 (TRPA-1) levels in type 2 diabetes mellitus (T2DM) mouse models induced with streptozocin (STZ) and a high-fat diet (HFD).

Materials and Methods: In this research, mice were allocated into six distinct groups: normal, negative control (HFD and STZ), positive control (metformin, HFD, and STZ), and three treatment groups (HFD, STZ, and Andaliman extract at varying dosages of 100, 300, and 500 mg/kg, respec­tively). Body weight and blood glucose levels (BGLs) were recorded at weeks 1 (baseline), 8, 12, and 16. The levels of TNF-α and TRPA-1 were measured during the 16th week.

Results: Phytochemical screening of the Andaliman extract revealed the presence of flavonoids, alkaloids, tannins, saponins, and glycosides. The one-way ANOVA revealed significantly elevated BGL at week 16 in the negative control group in comparison to the other groups (p < 0.05). The Kruskal-Wallis test followed by Bonferroni-corrected pairwise comparisons showed that the nega­tive control had significantly higher TNF-α levels than the Andaliman-groups (z = 22.11, p < 0.01). TRPA-1 was significantly higher in the negative control group compared to the treatment groups (p < 0.05). Furthermore, Spearman’s rho analysis revealed a statistically significant positive associ­ation between BGL and both TNF-α and TRPA-1, as well as between TNF-α and TRPA.

Conclusion: Andaliman extract potentially serves as a therapy for diabetic neuropathy in T2DM by lowering BGL and inhibiting the expression of TNF-α and TRPA-1.

Adv. Vet. Anim. Res., 11(2): 284-290, June 2024

http://doi.org/10.5455/javar.2024.k774

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Published

2024-06-06

How to Cite

Simbolon, B. M., Yulizal, O., Hutapea, A. M., & Handoko, E. (2024). The effect of Andaliman (Zanthoxylum acanthopodium DC.) fruit extracted with ethanol on TNF-α and TRPA-1 levels in type II diabetes-induced mice. Journal of Advanced Veterinary and Animal Research, 11(2), 284–290. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75670

Issue

Vol. 11 No. 2 (2024)

Section

Original Articles

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Copyright (c) 2024 Boyke Marthin Simbolon, OK Yulizal, Albert Manggading Hutapea, Erwin Handoko

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