Genetic variations of Toll-like receptor 4 gene in exon 2 of South African Dorper sheep

Authors

  • Lebelo Selala Department of Agricultural Economics and Animal Production, University of Limpopo, Polokwane, South Africa
  • Teedzai Chitura Department of Animal Science, University of Venda, Thohoyandou, South Africa
  • Vusi Mbazima Department of Biochemistry Microbiology and Biotechnology, University of Limpopo, Polokwane, South Africa
  • Louis Tyasi Department of Agricultural Economics and Animal Production, University of Limpopo, Polokwane, South Africa

Keywords:

DNA sequencing; genotypes; single nucleotide polymorphisms; Dorper sheep

Abstract

Objective: The study was conducted to identify the sequence variation of Toll-like receptor 4 (TLR4) in exon 2 of South African Dorper sheep.

Materials and Methods: Blood samples were collected from fifty (n = 50) South African Dorper sheep aged between 3 and 4 years. The Deoxyribonucleic acid (DNA) was extracted, amplified, and sequenced for the TLR4 gene. DNA sequencing was used to identify the sequence variations of the TLR4 gene in South African Dorper sheep.

Results: The results showed that one synonymous single nucleotide polymorphism (SNP) of the TLR4 gene in exon 2 position T2249C was identified. Two genotypes (TT and TC) were discovered from the identified SNP. The dominant genotype was TT (0.60) over TC (0.40), with the domi­nant allele T (0.80) over C (0.20). The results also indicated that the used population was in the Hady-Weinberg Equilibrium. Polymorphism genetic analysis findings suggest that the identified sequence variation of TLR4 in exon 2 of South African Dorper sheep was moderate polymorphism.

Conclusion: TLR4 gene at exon 2 of South African Dorper sheep had the SNP (T>C) at position 2249 bp with two genotypes (TT and TC).

Adv. Vet. Anim. Res., 11(2): 302-305, June 2024

http://doi.org/10.5455/javar.2024.k777

 

Downloads

Download data is not yet available.
Abstract
36
PDF
34

Downloads

Published

2024-06-08

How to Cite

Selala, L., Chitura, T., Mbazima, V., & Tyasi, L. (2024). Genetic variations of Toll-like receptor 4 gene in exon 2 of South African Dorper sheep. Journal of Advanced Veterinary and Animal Research, 11(2), 302–305. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75673

Issue

Vol. 11 No. 2 (2024)

Section

Short Communications

License

Copyright (c) 2024 Lebelo Selala, Teedzai Chitura, Vusi Mbazima, Louis Tyasi

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).