Genetic variations of Toll-like receptor 4 gene in exon 2 of South African Dorper sheep

Authors

  • Lebelo Selala Department of Agricultural Economics and Animal Production, University of Limpopo, Polokwane, South Africa
  • Teedzai Chitura Department of Animal Science, University of Venda, Thohoyandou, South Africa
  • Vusi Mbazima Department of Biochemistry Microbiology and Biotechnology, University of Limpopo, Polokwane, South Africa
  • Louis Tyasi Department of Agricultural Economics and Animal Production, University of Limpopo, Polokwane, South Africa

Keywords:

DNA sequencing; genotypes; single nucleotide polymorphisms; Dorper sheep

Abstract

Objective: The study was conducted to identify the sequence variation of Toll-like receptor 4 (TLR4) in exon 2 of South African Dorper sheep.

Materials and Methods: Blood samples were collected from fifty (n = 50) South African Dorper sheep aged between 3 and 4 years. The Deoxyribonucleic acid (DNA) was extracted, amplified, and sequenced for the TLR4 gene. DNA sequencing was used to identify the sequence variations of the TLR4 gene in South African Dorper sheep.

Results: The results showed that one synonymous single nucleotide polymorphism (SNP) of the TLR4 gene in exon 2 position T2249C was identified. Two genotypes (TT and TC) were discovered from the identified SNP. The dominant genotype was TT (0.60) over TC (0.40), with the domi­nant allele T (0.80) over C (0.20). The results also indicated that the used population was in the Hady-Weinberg Equilibrium. Polymorphism genetic analysis findings suggest that the identified sequence variation of TLR4 in exon 2 of South African Dorper sheep was moderate polymorphism.

Conclusion: TLR4 gene at exon 2 of South African Dorper sheep had the SNP (T>C) at position 2249 bp with two genotypes (TT and TC).

Adv. Vet. Anim. Res., 11(2): 302-305, June 2024

http://doi.org/10.5455/javar.2024.k777

 

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Published

2024-06-08

How to Cite

Selala, L., Chitura, T., Mbazima, V., & Tyasi, L. (2024). Genetic variations of Toll-like receptor 4 gene in exon 2 of South African Dorper sheep. Journal of Advanced Veterinary and Animal Research, 11(2), 302–305. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75673

Issue

Vol. 11 No. 2 (2024)

Section

Short Communications

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Copyright (c) 2024 Lebelo Selala, Teedzai Chitura, Vusi Mbazima, Louis Tyasi

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