Characterization of infectious laryngotracheitis virus isolated from commercial layer chickens in Bangladesh during the year 2021–2022

Authors

  • Md Mostofa Kamal Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Mohammad Sadekuzzaman Central Disease Investigation Laboratory, Department of Livestock Services, Dhaka, Bangladesh
  • Kohinoor Parvin Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Md Enamul Haque Department of Microbiology, Sheikh Hasina University of Science and Technology, Bhairab, Bangladesh
  • Sajedul Hayat Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Md Ariful Islam Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Mst Minara Khatun Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Mahbubul Pratik Siddique Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Sham Soun Nahar Livestock Research Institute, Mohakhali, Dhaka, Bangladesh
  • A K M Khasruzzaman Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Muhammud Tofazzal Hossain Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh
  • Md Alimul Islam Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh

Keywords:

Layer chickens; PCR; AGIDT; VNT; Mice; ITPI

Abstract

Objective: Infectious laryngotracheitis virus (ILTV) is responsible for causing infectious laryngo­tracheitis (ILT), which is a rapidly spreading and extremely transmissible disease in chickens. The current research aims to isolate and characterize ILTV from layer chickens in Bangladesh.

Materials and Methods: A total of 345 samples (trachea, larynx, and lungs) were collected from ILT-suspected dead and sick layer chickens of 32 ILT-suspected farms in three different outbreak districts (Gazipur, Tangail, and Mymensingh) of Bangladesh during the outbreak year 2021-2022. Rapid detection kits examined the samples for avian influenza virus (AIV) and Newcastle disease virus (NDV). ILTV-specific primers were used to screen 72 NDV- and AIV-negative samples by poly­merase chain reaction (PCR). Using chorioallantoic membrane (CAM), the study isolated the ILT virus from 9 to 10-day-old seronegative embryonated chicken eggs (ECEs) using selected PCR-positive samples. The virus was confirmed using nucleotide sequencing, agar gel immunodiffusion test (AGIDT), viral neutralization test (VNT), and pathogenicity evaluations using mortality index for chicken embryos (MICEs) and intra-tracheal pathogenicity index (ITPI).

Results: The results indicated that among the PCR-positive 10 samples, only two (Alim_ILT_1001 and Alim_ILT_1,000) were found positive using ECEs. There were two field isolates of ILTVs, as shown by the amplicon size of the ICP4 gene-based PCR. A phylogenetic study of the ICP4 gene revealed that the recent isolates have a close similarity with the ILTV isolates of Turkey, Bangladesh, and Australia. AGIDT revealed strong precipitation lines due to ILTV-specific antibod­ies reacting with field viruses, while VNT neutralized both isolates with conventional ILTV antibod­ies. The pathogenicity testing indicated that Alim_ILT_1001 had MICE and ITPI values of 0.77 and 0.63, whereas Alim_ILT_1,000 had 0.71 and 0.57.

Conclusion: Both the ILTV isolates have similarities with the isolates of Turkey, Bangladesh, and Australia, and they are highly virulent for chickens.

Adv. Vet. Anim. Res., 11(2): 398-407, June 2024

http://doi.org/10.5455/javar.2024.k789

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Published

2024-08-29

How to Cite

Kamal, M. M., Sadekuzzaman, M., Parvin, K., Haque, M. E., Hayat, S., Islam, M. A., … Islam, M. A. (2024). Characterization of infectious laryngotracheitis virus isolated from commercial layer chickens in Bangladesh during the year 2021–2022. Journal of Advanced Veterinary and Animal Research, 11(2), 398–407. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75720

Issue

Vol. 11 No. 2 (2024)

Section

Original Articles

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Copyright (c) 2024 Md Mostofa Kamal, Mohammad Sadekuzzaman, Kohinoor Parvin, Md Enamul Haque, Sajedul Hayat, Md Ariful Islam, Mst Minara Khatun, Mahbubul Pratik Siddique, Sham Soun Nahar, A K M Khasruzzaman, Muhammud Tofazzal Hossain, Md Alimul Islam

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