Antibiogram profiling and detection of icaA and blaZ genes from Staphylococcus aureus and coagulase-negative Staphylococcus spp. of healthy bovine raw milk sample origin

Authors

  • Asmaul Husna Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh Bangladesh
  • Md Arefin Kallol Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh Bangladesh
  • Farhana Binte Ferdous Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh Bangladesh
  • Khudaza Akter Lima Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh Bangladesh
  • Zannatul Haque Tumpa Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh Bangladesh
  • Mohammad Ferdousur Rahman Khan Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh Bangladesh
  • Marzia Rahman Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh Bangladesh

Keywords:

blaZ; CoNS; icaA; MDR; Biofilm; S. aureus.

Abstract

Objective: This study focused on the antibiogram profiling of Staphylococcus aureus and coagu­lase-negative Staphylococcus spp. (CoNS) and the detection of icaA and blaZ genes from bovine raw milk samples.

Materials and Methods: Bovine milk samples were collected from dairy farms, and Staphylococcus spp. were isolated and identified via conventional and molecular screening. Disk diffusion test (DDT) was implemented to determine the resistance pattern. Biofilm and β-lactamase-produc­ing Staphylococcus spp. were identified via amplification of the icaA and blaZ genes. Methicillin-resistant Staphylococcus aureus and CoNS were identified by DDT and PCR of the mecA gene.

Results: From 63 samples, 35 were confirmed as Staphylococcus spp., of which 16 (25.39%) S. aureus isolates were coagulase-positive, while 19 (30.16%) were negative. PCR confirmed that 50% (8/16) of S. aureus and 36.84% (7/19) of CoNS possessed the icaA gene. All S. aureus iso­lates were found resistant to penicillin-G (P) both phenotypically and genotypically. The isolates were also resistant to erythromycin (ERY) and oxytetracycline (TET). While CoNS showed high to reduced resistance against P, TET, ERY, and azithromycin, no S. aureus isolates were resistant to sulfamethoxazole, while 10.53% of CoNS isolates were. All S. aureus and CoNS isolates were sus­ceptible to vancomycin and gentamicin. MR was exhibited by 37.5% of S. aureus and 42.10% of CoNS isolates. Moreover, S. aureus and CoNS had 56.25% and 52.63% multidrug-resistant (MDR) isolates, respectively.

Conclusion: The present study revealed the presence of a biofilm-producing, MDR staphylococcal strain in milk that might endanger consumers. Routine surveillance and monitoring, along with antimicrobial resistance learning, can reduce risks.

Adv. Vet. Anim. Res., 11(2): 455-462, June 2024

http://doi.org/10.5455/javar.2024.k795

 

Downloads

Download data is not yet available.
Abstract
37
PDF
29

Downloads

Published

2024-06-19

How to Cite

Husna, A., Kallol, M. A., Ferdous, F. B., Lima, K. A., Tumpa, Z. H., Rahman Khan, M. F., & Rahman, M. (2024). Antibiogram profiling and detection of icaA and blaZ genes from Staphylococcus aureus and coagulase-negative Staphylococcus spp. of healthy bovine raw milk sample origin. Journal of Advanced Veterinary and Animal Research, 11(2), 455–462. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75739

Issue

Vol. 11 No. 2 (2024)

Section

Original Articles

License

Copyright (c) 2024 Asmaul Husna

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).